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- 23Chemistry, Department of/Journal Articles (Chemistry)
- 5Graduate and Postdoctoral Studies (GPS), Faculty of
- 5Graduate and Postdoctoral Studies (GPS), Faculty of /Theses and Dissertations
- 1Biological Sciences, Department of
- 1Biological Sciences, Department of/Journal Articles (Biological Sciences)
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2014
Marcondes, Maria C. G., Macauley, Matthew S., Pang, Poh-Choo, Bortell, Nikki, Arlian, Britni M., Rillahan, Cory D., Haslam, Stuart M., Dell, Anne , Paulson, James C.
Sialic acid terminates glycans of glycoproteins and glycolipids that play numerous biological roles in health and disease. Although genetic tools are available for interrogating the effects of decreased or abolished sialoside expression in mice, pharmacological inhibition of the sialyltransferase...
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Study of Non-Covalent Protein-Carbohydrate Interactions using Electrospray Ionization Mass Spectrometry
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This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) methods to study protein-carbohydrate interactions in vitro. The affinities (Ka) of two recombinant fragments of Clostridium difficile toxins (TcdA & TcdB) and a library of the most...
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Reliable determinations of protein-ligand interactions by direct ESI-MS measurements: Are we there yet?
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El-Hawiet, Amr, Klassen, John S., Kitova, Elena N., Schnier, Paul D.
The association-dissociation of noncovalent interactions between protein and ligands, such as other proteins, carbohydrates, lipids, DNA, or small molecules, are critical events in many biological processes. The discovery and characterization of these interactions is essential to a complete...
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Reliable determinations of protein-ligand interactions by direct ESI-MS measurements: Are we there yet?
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Klassen, John S., Kitova, Elena N., Schnier, Paul D., El-Hawiet, Amr
The association-dissociation of noncovalent interactions between protein and ligands, such as other proteins, carbohydrates, lipids, DNA, or small molecules, are critical events in many biological processes. The discovery and characterization of these interactions is essential to a complete...
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2015
El-Hawiet, Amr, Klassen, John S., N. Kitova, Elena N.
The affinities of the most abundant oligosaccharides found in human milk for four bacterial exotoxins (from Vibrio cholerae and pathogenic Escherichia coli) were quantified for the first time. Association constants (Ka) for a library of 20 human milk oligosaccharides (HMOs) binding to Shiga toxin...
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2015
El-Hawiet, Amr, N. Kitova, Elena N., Klassen, John S.
The affinities of the most abundant oligosaccharides found in human milk for four bacterial exotoxins (from Vibrio cholerae and pathogenic Escherichia coli) were quantified for the first time. Association constants (Ka) for a library of 20 human milk oligosaccharides (HMOs) binding to Shiga toxin...
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Quantitative Characterization of Noncovalent Protein-Carbohydrate Interactions using Electrospray Ionization Mass Spectrometry
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The interactions between water-soluble proteins and carbohydrates found on the surfaces of cells play important roles in many physiological and pathological cellular processes. Carbohydrates function as receptors for signaling, cellular recognition and adhesion, and pathogen infections. This...
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Quantifying protein-fatty acid interactions using electrospray ionization mass spectrometry
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Liu, Lan, Kitova, Elena N., Klassen, John S.
The application of the direct electrospray ionization mass spectrometry (ESI-MS) assay to quantify interactions between bovine β-lactoglobulin (Lg) and a series of fatty acids (FA), CH3(CH2)xCOOH, where x = 6 (caprylic acid, CpA), 8 (capric acid, CA), 10 (lauric acid, LA), 12 (myristic acid, MA),...
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Quantifying protein-fatty acid interactions using electrospray ionization mass spectrometry
Download2011
Klassen, John S., Kitova, Elena N., Liu, Lan
The application of the direct electrospray ionization mass spectrometry (ESI-MS) assay to quantify interactions between bovine β-lactoglobulin (Lg) and a series of fatty acids (FA), CH3(CH2)xCOOH, where x = 6 (caprylic acid, CpA), 8 (capric acid, CA), 10 (lauric acid, LA), 12 (myristic acid, MA),...
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2015
Klassen, John S., Darestani, Reza Rezaei, Kitova, Elena N., Zou, Chunxia, Li, Jun, Leney, Aneika C., Cairo, Christopher W.Xiong, Zi Jian, Privé, Gilbert G., Nikjah, Sanaz
Protein interactions with glycolipids are implicated in diverse cellular processes. However, the study of protein–glycolipid complexes remains a significant experimental challenge. Here, we describe a powerful new assay that combines electrospray ionization mass spectrometry (ESI-MS) and...