SearchSkip to Search Results
- 3Electrospray ionization
- 3Isotope labeling
- 3Liquid chromatography
- 1Alexander, Anthony John.
- 1Allen, Felicity R
- 1Black, Brenna A
- 1Boyd, Jessica M.
- 1Chen, Lu
- 1Dawe, Margot Renee
This thesis describes the application of mass spectrometry (MS) to glycoprotein and oligosaccharide analysis. Glycosylated proteins are involved in cell-cell and cell-matrix recognition. Applications of trypsin and proteinase K to hydrolyze glycoproteins into glycopeptides that are compatible...
Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl MetabolomeDownload
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...
The proteome, a hybrid term from protein and genome, is the total set of proteins expressed by a genome in a cell, tissue, or organism at a given time. Proteomics, the study of the proteome, is concerned with a variety of topics such as identity, quantity, activity, structure, protein-protein...
Development of Liquid Chromatography Mass Spectrometry Methods for the Identification and Quantification of Acylcarnitines in Biological SamplesDownload
The field of metabolomics follows the Greek premise where metabolic changes are believed to be indicative of disease. Acylcarnitines, for example, can be dysregulated in the presence of various diseases including genetic metabolic disorders and multiple sclerosis. Liquid chromatography mass...
Liquid crystalline domains were observed in fractions of heavy petroleum. Through a combination of polarized light microscopy and Photoacoustic Infrared Spectroscopy, liquid crystals were shown to form on the exterior surface of their parent materials. Analysis of materials using Differential...
Development of Isotope Labeling Liquid Chromatography Mass Spectrometry for Biofluid Metabolomics and Applications in Disease StudiesDownload
Metabolomics is a research field focusing on global study of all the metabolites present in a biological system (i.e., the metabolome). Metabolome analysis involves identifying and quantifying as many small molecule metabolites as possible in a biological sample. Metabolomics has attracted much...
One of the key obstacles to the effective use of mass spectrometry (MS) in high throughput metabolomics is the difficulty in interpreting measured spectra to accurately and efficiently identify metabolites. Traditional methods for automated metabolite identification compare the target MS spectrum...
Application of Electrospray Ionization Mass Spectrometry to Study Protein-Ligand Interactions and Enzyme KineticsDownload
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) to study the noncovalent protein-ligand interactions and also investigate the kinetic of enzymatic reactions. The rate of glycolipids cleavage by the human neuraminidase 3 (hNEU3) was...
Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate InteractionsDownload
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer...