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Huh7.5 Cells Grown in Human Serum as a Model to Study the Effects of Hepatitis C Virus Infection on Lipid CatabolismDownload
Our goal is to study the additional elements of the mechanism of steatosis underlying hepatitis C virus (HCV) infection. Human hepatoma Huh7.5 cells cultured in human serum (HS) were used as a model to study the development of steatosis. Huh7.5 cells grown in HS media were chosen to study the...
Type-1 Brassica napus Diacylglycerol Acyltransferases: Enzyme Characterization and Molecular Tools for Increasing Storage Lipid Production in YeastDownload
Diacylglycerol acyltransferase (DGAT) catalyzes the final step in the acyl-CoA- dependent biosynthesis of triacylglycerol (TAG). The level of DGAT activity may have a substantial effect on the flow of carbon into TAG in many organisms. In plants, yeast, and animals, two families of...
Plant acyl-CoA:lysophosphatidylcholine acyltransferases (LPCATs) have different specificities in their forward and reverse reactionsDownload
Heinz, Ernst, Zhou, Xue-Rong, Banas, Antoni, Green, Allan, Jasieniecka, Katarzyna, Weselake, Randall, Smith, Mark A., Stymne, Sten, Bayon, Shen, Zou, Jitao, Lager, Ida, Dyer, John M., Shockey, Jay M., Yilmaz, Jenny L., Wang, Peng, Kazachkov, Michael
Acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) enzymes have central roles in acyl editing of phosphatidylcholine (PC). Plant LPCAT genes were expressed in yeast and characterized biochemically in microsomal preparations of the cells. Specificities for different acyl-CoAs were similar...
Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinoleninDownload
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
Elucidation of molecular mechanisms of physiological variations between bovine subcutaneous and visceral fat depots under different nutritional regimesDownload
Adipose tissue plays a critical role in energy homeostasis and metabolism. There is sparse understanding of the molecular regulation at the protein level of bovine adipose tissues, especially within different fat depots under different nutritional regimes. The objective of this study was to...
Altered microRNA expression in bovine subcutaneous and visceral adipose tissues from cattle under different dietDownload
Background MicroRNAs (miRNAs) are a class of molecular regulators found to participate in numerous biological processes, including adipogenesis in mammals. This study aimed to evaluate the differences of miRNA expression between bovine subcutaneous (backfat) and visceral fat depots (perirenal...
Background Optimal pancreatic β-cell function is essential for the regulation of glucose homeostasis in both humans and animals and its impairment leads to the development of diabetes. Type 2 diabetes is a polygenic disease aggravated by environmental factors such as low physical activity or a...