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Study of Non-Covalent Protein-Carbohydrate Interactions using Electrospray Ionization Mass Spectrometry
Download2012-11
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) methods to study protein-carbohydrate interactions in vitro. The affinities (Ka) of two recombinant fragments of Clostridium difficile toxins (TcdA & TcdB) and a library of the most...
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Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate Interactions
Download2016-06
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer...
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