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High resolution radiation hybrid maps of bovine chromosome 19 and 29 and comparison with the bovine genome sequence assemblyDownload
Background High resolution radiation hybrid (RH) maps can facilitate genome sequence assembly by correctly ordering genes and genetic markers along chromosomes. The objective of the present study was to generate high resolution RH maps of bovine chromosomes 19 (BTA19) and 29 (BTA29), and...
Schnabel, R. D., Wang, Z., Mannenl, H., Gill, C. A., Van Tassell, C. P., Gao, C., Aerts, J., Williams, J. L., Neto, E. D., Moore, S. S., Matukumalli, L. K., Coppieters, W., McKay, S. D., Taylor, J. F., Crews, D., Murdoch, B. M.
Background Bovine whole genome linkage disequilibrium maps were constructed for eight breeds of cattle. These data provide fundamental information concerning bovine genome organization which will allow the design of studies to associate genetic variation with economically important traits and...
Expansion of ruminant-specific microRNAs shapes target gene expression divergence between ruminant and non-ruminant speciesDownload
Background Understanding how species-specific microRNAs (miRNAs) contribute to species-specific phenotypes is a central topic in biology. This study aimed to elucidate the role of ruminant-specific miRNAs in shaping mRNA expression divergence between ruminant and non-ruminant species. ...
A 3.8 kb male specific fragment was observed following agarose gel etectrophoresis of porcine DNA digested with Sph I. DNA was recovered from this region of a gel using DE-81 paper and ligated to Sph I digested pUC18. Plasmids carrying male specific sequences were identified by their differential...