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Skip to Search Results- 13Cryopreservation
- 4Vitrification
- 3Articular cartilage
- 3Chondrocytes
- 3Cryobiology
- 3Cryoprotectant agents
- 9Chemical and Materials Engineering, Department of
- 9Chemical and Materials Engineering, Department of/Research Articles and Materials (Chemical and Materials Engineering)
- 5Graduate and Postdoctoral Studies (GPS), Faculty of
- 5Graduate and Postdoctoral Studies (GPS), Faculty of /Theses and Dissertations
- 4Surgery, Department of
- 4Surgery, Department of/Orthopaedic Surgery
- 1Elliott, Janet A. W. (Chemical & Materials Engineering and Laboratory Medicine & Pathology)
- 1Elliott, Janet A.W. (Chemical and Materials Engineering)
- 1Elliott, Janet AW (Chemical and Materials Engineering)
- 1Jomha, Nadr (Surgery)
- 1Kumar, Piyush (Oncology)
- 1McGann, Locksley E (Laboratory Medicine and Pathology)
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Versatile Polymer-Based Nanomedicines for Drug/Gene Delivery and Cell-based Therapy Applications
DownloadSpring 2021
Advances in polymer science and nanotechnology have allowed the development of biomaterials for cell-based applications, such as the delivery of a variety of drugs and genes, as well as supporting technologies, such as stem cell therapies, tissue engineering and cryopreservation. The progress in...
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Fall 2015
Cryopreserving cells for human health therapeutics is a multidisciplinary process with great complexity. Cryopreservation of human umbilical vein endothelial cells (HUVECs) has facilitated vascular biology research since they were first isolated in 1973 [59]; however identifying key variables to...
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2014-09-26
Rekieh, K., Abazari, A., Elliott, J.A., Jomha, N.M., Law, G.K., McGann, L.E.
Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC) could improve the clinical availability...
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Fall 2015
Osteoarthritis, a disease resulting in the breakdown of cartilage and bone within joints, is a global burden that is growing in scope. There is no cure for osteoarthritis, and the current treatments are all lacking in some form or another. One treatment which attempts to prevent degeneration of...
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Modeling the transport of cryoprotective agents in articular cartilage for cryopreservation
DownloadSpring 2011
Loading vitrifiable concentrations of cryoprotective agents is an important step for cryopreservation of biological tissues by vitrification for research and transplantation purposes. This may be done by immersing the tissue in a cryoprotective agent (CPA) solution, and increasing the...
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Investigating cryoinjury using simulations and experiments: 2. TF-1 cells during graded freezing (interrupted slow cooling without hold time)
Download2014-09-22
McGann, L.E., Ross-Rodriguez, L.U., Elliott, J.A.
Cryopreservation plays a key role in the long-term storage of native and engineered cells and tissues for research and clinical applications. The survival of cells and tissues after freezing and thawing depends on the ability of the cells to withstand a variety of stresses imposed by the...
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Investigating cryoinjury using simulations and experiments: 1. TF-1 cells during two-step freezing (rapid cooling interrupted with a hold time)
Download2014-09-22
McGann, L. E., Ross-Rodriguez, L. U., Elliott, J. A.
There is significant interest in designing a cryopreservation protocol for hematopoietic stem cells (HSC) which does not rely on dimethyl sulfoxide (Me2SO) as a cryoprotectant. Computer simulations that describe cellular osmotic responses during cooling and warming can be used to optimize the...
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2014
Prasad, V., Laouar, L., Jomha, N. M., McGann, L. E., Fahmy, M. D., Almansoori, K. A., Elliott, J. A.
Vitrification of articular cartilage (AC) could enhance tissue availability but requires high concentrations of cyroprotective agents (CPAs). This study investigated relative injuries caused by commonly used CPAs. We hypothesized that the in situ chondrocyte dose–injury relationships of five...
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2012
Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.
Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes. However, at...