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- 1Alford, Spencer Caleb
- 1Anbukarasu, Preetam
- 1Arutyunov, Denis
- 1Assempour, Nazanin
- 1Belal, Ahmed S. F.
- 1Carlson, Haley J
Nanomechanical resonators offer a pathway towards highly sensitive and label-free detection of biomolecules by transducing the mass of bound analytes into resonant frequency shifts. Zeptogram level and even single cell detections have been successfully achieved by others. However, these...
Fluorescent proteins (FP) and FP-based biosensors have become essential tools for cell biology and neuroscience research. This thesis describes efforts to engineer new FPs with red emission and novel genetically encoded biosensors based on red FPs. Directed evolution and semi-rational design are...
The discovery of green fluorescent protein (GFP) from the Aequorea victoria jellyfish revolutionized many fields in the scientific community, including molecular biology, protein engineering, and neuroscience. The ability to genetically link a fluorescent protein to a protein of interest has...
Microcantilever sensor has gained much popularity because of its high sensitivity and selectivity. It consists of a micro-sized cantilever that is usually coated on one side with chemical/biological probe agents to generate strong attraction to target molecules. The interactions between the probe...
Development of an optical biosensor based on Mach-Zehnder Interferometry for detection of Listeria monocytogenesDownload
Label-free optical biosensors are widely used for the detection of pathogens due to their low-cost, real-time detection and high sensitivity. A Mach-Zehnder Interferometer (MZI) based biosensor has been developed for the detection of gram-positive bacteria, Listeria monocytogenes. An extensive...
The discovery and subsequent applications of fluorescent proteins (FPs) launched a new era for live cell fluorescence imaging. The design and developments of FP-based indicators have further solidified the versatility of FPs and rendered them as indispensable tools in life science research now...
Resonant microcantilevers are promising transducers for bacteria detection because of their high sensitivities. Surface stress and mass from adsorbates affect the resonant frequency. We developed a novel method for decoupling the frequency contributions of a change in mass and surface stress on a...
The field of conducting polymers and mainly organic electrochemical transistors (OECT) have been investigated for bioelectronics applications. Advantages such as conformability, high quality electrical contact, ease of fabrication and biocompatibility made OECTs a great potential of future bio...
Specific detection of Campylobacter jejuni using the bacteriophage NCTC 12673 receptor binding protein as a probeDownload
Campylobacter jejuni is found in the intestines of poultry, cattle, swine, wild birds and pet animals and is the major cause of foodborne gastroenteritis in developed countries. We report the use of the receptor binding protein (RBP) of Campylobacter bacteriophage NCTC 12673 for the specific...