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- 3Mietkiewska, Elzbieta
- 3Siloto, Rodrigo M. P.
- 3Weselake, Randall J.
- 2Pan, Xue
- 2Wickramarathna, Aruna D.
- 1Caldo, Kristian Mark P.
- 1Brassica napus
- 1Diacylglycerol acyltransferase
- 1Directed evolution
- 1Leaf oil production
Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinoleninDownload
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure functionDownload
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seedsDownload
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....