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Comparative genomics of Lactobacillus reuteri from sourdough reveals adaptation of an intestinal symbiont to food fermentations
Download2015
Zhao, Xin, Gänzle, Michael G., Lin, Xiaoxi B., Zheng, Jinshui
Lactobacillus reuteri is a dominant member of intestinal microbiota of vertebrates, and occurs in food fermentations. The stable presence of L. reuteri in sourdough provides the opportunity to study the adaptation of vertebrate symbionts to an extra-intestinal habitat. This study evaluated this...
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Diet and environment shape fecal bacterial microbiota composition and enteric pathogen load of grizzly bears
Download2011-01-01
Northrup, Joseph M., Cristescu, Bogdan, Schwab, Clarissa, Stenhouse, Gordon B., Gänzle, Michael G.
Background Diet and environment impact the composition of mammalian intestinal microbiota; dietary or health disturbances trigger alterations in intestinal microbiota composition and render the host susceptible to enteric pathogens. To date no long term monitoring data exist on the fecal...
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Effect of chitosan, and bacteriocin – Producing Carnobacterium maltaromaticum on survival of Escherichia coli and Salmonella Typhimurium on beef
Download2018-01-01
Hu, Zi Yi, Balay, Danielle, Hu, Ying, McMullen, Lynn M., Gänzle, Michael G.
The aim of this study was to investigate the synergistic effect of chitosan and bacteriocins against Escherichia coli and Salmonella in media and in lean beef. The inhibitory effects of chitosan and bacteriocins against E. coli AW1.7 and S. enterica Typhimurium in media were determined by a...
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Comparative assessment of qPCR enumeration methods that discriminate between live and dead Escherichia coli O157:H7 on beef
Download2018-01-01
Laidlaw, Anna M., Gänzle, Michael G., Yang, Xianqin
Quantitative Polymerase Chain Reaction (qPCR) is a molecular method commonly used to detect and quantify bacterial DNA on food but is limited by its inability to distinguish between live and dead cell DNA. To overcome this obstacle, propidium monoazide (PMA) alone or with deoxycholate (DC) was...
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Inulin and fructo-oligosaccharides have divergent effects on colitis and commensal microbiota in HLA-B27 transgenic rats
Download2012-01-01
Koleva, Petya, Dieleman, Levinus, Sun, Xu, Gänzle, Michael G., Valcheva, Rosica
Modulation of intestinal microbiota by non-digestible carbohydrates may reduce inflammation in inflammatory bowel disease (IBD). The aim of the present study was to assess the effects of inulin and fructo-oligosaccharides (FOS) on intestinal microbiota and colitis in HLA-B27 transgenic rats, a...
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Contribution of glutaminases to glutamine metabolism and acid resistance in Lactobacillus reuteri and other vertebrate host adapted lactobacilli
Download2020-04-01
Li, Qing, Tao, QianYing, Teixeira, Juanana S., Shu-Wei Su, Marcia, Gänzle, Michael G.
The bacterial conversion of glutamine to glutamate is catalyzed by glutamine-amidotransferases or glutaminases. Glutamine deamination contributes to the formation of the bioactive metabolites glutamate, γ-aminobutyrate (GABA) and γ-glutamyl peptides, and to acid resistance. This study aimed to...
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Sensory analysis of juice blend containing isomalto-oligosaccharides produced by fermentation with Weissella cibaria
Download2019-01-01
Rolim, Pricilla Moura, Hu, Ying, Gänzle, Michael G.
This study aimed at producing isomaltooligosaccharides in juice blends using orange juice and malt extract and assessing their acceptability. Different blend formulations were prepared and fermented, varying the concentration of orange juice, sucrose and malt extract. Dextransucrase from...
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2016-01-01
Li, Hui, Garcia-Hernandez, Rigoberto, Driedger, Darcy, McMullen, Lynn M., Gänzle, Michael G.
The pressure resistance of Shiga-toxin producing Escherichia coli (STEC) depends on food matrix. This study compared the resistance of two five-strain E. coli cocktails, as well as the pressure resistant strain E. coli AW1.7, to hydrostatic pressure application in bruschetta, tzatziki, yoghurt...
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Characterization of α-Galactooligosaccharides formed via Heterologous Expression of α- 2 Galactosidases from Lactobacillus reuteri in Lactococcus lactis
Download2014-01-01
Wang, Yvonne, Black, Brenna A., Curtis, Jonathan M., Gänzle, Michael G.
α-Galacto-oligosaccharides (α-GOS) are produced by transgalactosylation reactions of α-galactosidase (α-Gal) or by conversion of raffinose family oligosaccharides by levansucrase. Similarly to β-GOS, α-GOS have the potential to mimic glycan receptors on eukaryotic cells and act as molecular...
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Host-adapted lactobacilli in food fermentations: impact of metabolic traits of host adapted lactobacilli on food quality and human health
Download2020-10-27
Back-slopping of fermentation cultures in food fermentations can ensure stability of fermentation microbiota at the species or even at the strain level over extended periods of time. In contrast to the fermentation organisms in spontaneous food fermentations, which are derived from...