Search
Skip to Search Results- 31Chen, Guanqun
- 17Xu, Yang
- 15Singer, Stacy D.
- 14Weselake, Randall J.
- 9Caldo, Kristian Mark P.
- 7Mietkiewska, Elzbieta
- 9DGAT
- 6Triacylglycerol biosynthesis
- 5Saccharomyces cerevisiae
- 4Triacylglycerol
- 3Arabidopsis thaliana
- 3LACS
-
2020-11-01
Xu, Yang, Mietkiewska, Elzbieta, Sha, Saleh, Weselake, Randall J., Chen, Guanqun
Punicic acid (PuA; 18:3Δ9cis,11trans,13cis), a conjugated linolenic acid isomer bearing three conjugated double bonds, is associated with various health benefits and has potential for industrial use. The major nature source of this unusual fatty acid is pomegranate (Punica granatum) seed oil,...
-
Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein
Download2020-01-01
Xu, Yang, Caldo, Kristian Mark P., Falarz, Lucas, Jayawardhane, Kethmi, Chen, Guanqun
SUMMARY Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes the final step of acyl-CoA-dependent biosynthesis of TAG and is considered a key target for manipulating oil production....
-
The potential of genome editing for improving seed oil content and fatty acid composition in oilseed crops
Download2020-08-27
Subedi, Udaya, Jayawardhane, Kethmi N., Pan, Xue, Ozga, Jocelyn, Chen, Guanqun, Foroud, Nora A., Singer, Stacy D.
A continuous rise in demand for vegetable oils, which comprise mainly the storage lipid triacylglycerol, is fueling a surge in research efforts to increase seed oil content and improve fatty acid composition in oilseed crops. Progress in this area has been achieved using both conventional...
-
2020-03-01
Tian, Bo, Sun, Meijuan, Jayawardana, Kethmi, Wu, Ding, Chen, Guanqun
Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri‐ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n‐9) esterified to the sn‐2 position of phosphatidylcholine (PtdCho) catalyzed by...
-
Enhancement of total lipid production in vegetative tissues of alfalfa and sainfoin using chemical mutagenesis
Download2019-12-31
Wijekoon, Champa P., Singer, Stacy D., Weselake, Randall J., Petrie, James R., Singh, Surinder, Jayawardhane, Kethmi N., Shah, Saleh, Chen, Guanqun, Eastmond, Peter J., Acharya, Surya N.
Alfalfa (Medicago sativa L.) and sainfoin (Onobrychis viciifoila Scop.) are two key forage legumes for the western Canadian cattle industry. Despite the high protein content, drawbacks to their use exists, including inefficient protein digestibility and energy use efficiency in the ruminant...
-
Metabolomic study of stress responses leading to plant resistance in mandarin fruit mediated by preventive applications of Bacillus subtilis cyclic lipopeptides
Download2019-10-01
Tunsagool, Paiboon, Wang, Xiohang, Leelasuphakul, Wichitra, Jutidamrongphan, Warangkana, Phaonakrop, Narumon, Jaresitthikunchai, Janthima, Roytrakul, Sittiruk, Chen, Guanqun, Li, Liang
Although green mold rot caused by Penicillium digitatum is a major postharvest disease in mandarin fruit, the fruit’s defense mechanism at the metabolomic level is largely unknown. Here, the expressed metabolome network leading to plant resistance to stresses induced by exposing of different...
-
A Fluorescence‐Based Assay for Quantitative Analysis of Phospholipid:Diacylglycerol Acyltransferase Activity
Download2019-09-02
Falarz, Lucas, Xu, Yang, Singer, Stacy D., Chen, Guanqun
Phospholipid:diacylglycerol acyltransferase (PDAT) catalyzes the acyl‐CoA‐independent triacylglycerol (TAG) biosynthesis in plants and oleaginous microorganisms and thus is a key target in lipid research. The conventional in vitro PDAT activity assay involves the use of radiolabeled substrates,...
-
Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme
Download2019-12-26
Nguyen, Trinh, Xu, Yang, Abdel-Hameed, Mona, Sorensen, John L., Singer, Stacy D., Chen, Guanqun
Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...
-
Characterization of Type-2 Diacylglycerol Acyltransferases in the Green Microalga Chromochloris zofingiensis
Download2018-12-13
Xu, Yang, Falarz, Lucas, Chen, Guanqun
Diacylglycerol acyltransferase (DGAT) catalyzes the last and committed step of the acyl-CoA-dependent TAG biosynthesis and thus is a key target for manipulating oil production in microalgae. The microalga Chromochloris zofingiensis can accumulate substantial amounts of triacylglycerol (TAG) and...
-
Plant sn‐Glycerol‐3‐Phosphate Acyltransferases: Biocatalysts Involved in the Biosynthesis of Intracellular and Extracellular Lipids
Download2018-05-31
Jayawardhane, Kethmi N., Singer, Stacy D., Weselake, Randall J., Chen, Guanqun
Acyl‐lipids such as intracellular phospholipids, galactolipids, sphingolipids, and surface lipids play a crucial role in plant cells by serving as major components of cellular membranes, seed storage oils, and extracellular lipids such as cutin and suberin. Plant lipids are also widely used to...