Search
Skip to Search Results- 31Chen, Guanqun
- 17Xu, Yang
- 15Singer, Stacy D.
- 14Weselake, Randall J.
- 9Caldo, Kristian Mark P.
- 7Mietkiewska, Elzbieta
- 9DGAT
- 6Triacylglycerol biosynthesis
- 5Saccharomyces cerevisiae
- 4Triacylglycerol
- 3Arabidopsis thaliana
- 3LACS
-
Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to enrichment of flax seed oil with α -linolenic acid
Download2018-01-01
Xu, Yang, Holic, Roman, Li, Darren, Mietkiewska, Elzbieta, Chen, Guanqun, Ozga, Jocelyn, Weselake, Randall
Seed oil from flax ( Linum usitatissimum ) is enriched in α -linolenic acid (ALA; 18:3 Δ 9 cis ,12 cis ,15 cis) but the biochemical processes underlying the enrichment of flax seed oil with this polyunsaturated fatty acid are not fully elucidated. Here, a potential process involving the catalytic...
-
Properties and Biotechnological Applications of Acyl‐CoA:diacylglycerol Acyltransferase and Phospholipid:diacylglycerol Acyltransferase from Terrestrial Plants and Microalgae
Download2018-07-30
Xu, Yang, Caldo, Kristian Mark P., Pal‐Nath, Dipasmita, Ozga, Jocelyn, Lemieux, M. Joanne, Weselake, Randall J., Chen, Guanqun
Triacylglycerol (TAG) is the major storage lipid in most terrestrial plants and microalgae, and has great nutritional and industrial value. Since the demand for vegetable oil is consistently increasing, numerous studies have been focused on improving the TAG content and modifying the fatty‐acid...
-
Identification of genes associated with ricinoleic acid accumulation in Hiptage benghalensis via transcriptome analysis
Download2018-12-16
Tian, Bo, Lu, Tianquan, Xu, Yang, Wang, Ruling, Chen, Guanqun
Background: Ricinoleic acid is a high-value hydroxy fatty acid with broad industrial applications.Hiptage benghalensis seed oil contains a high amount of ricinoleic acid (~80%) and represents an emerging source of this unusual fatty acid. However, the mechanism of ricinoleic acid accumulation in...
-
2018-03-03
Holic, Roman, Xu, Yang, Caldo, Kristian Mark P., Singer, Stacy D., Field, Catherine J., Weselake, Randall J., Chen, Guanqun
Punicic acid (PuA; 18: 3Δ 9cis,11trans,13cis ) is an unusual 18-carbon fatty acid bearing three conjugated double bonds. It has been shown to exhibit a myriad of beneficial bioactivities including anti-cancer, anti-diabetes, anti-obesity, antioxidant, and anti-inflammatory properties. Pomegranate...
-
Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein
Download2020-01-01
Xu, Yang, Caldo, Kristian Mark P., Falarz, Lucas, Jayawardhane, Kethmi, Chen, Guanqun
SUMMARY Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes the final step of acyl-CoA-dependent biosynthesis of TAG and is considered a key target for manipulating oil production....
-
Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to enrichment of flax seed oil with a-linolenic acid
Download2018-04-01
Xu, Yang, Holic, Roman, Li, Darren, Pan, Xue, Mietkiewska, Elzbieta, Chen, Guanqun, Ozga, Jocelyn, Weselake, Randall J.
Seed oil from flax (Linum usitatissimum) is enriched in a-linolenic acid (ALA; 18:3?9cis,12cis,15cis), but the biochemical processes underlying the enrichment of flax seed oil with this polyunsaturated fatty acid are not fully elucidated. Here, a potential process involving the catalytic actions...
-
The potential of genome editing for improving seed oil content and fatty acid composition in oilseed crops
Download2020-08-27
Subedi, Udaya, Jayawardhane, Kethmi N., Pan, Xue, Ozga, Jocelyn, Chen, Guanqun, Foroud, Nora A., Singer, Stacy D.
A continuous rise in demand for vegetable oils, which comprise mainly the storage lipid triacylglycerol, is fueling a surge in research efforts to increase seed oil content and improve fatty acid composition in oilseed crops. Progress in this area has been achieved using both conventional...
-
Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme
Download2019-12-26
Nguyen, Trinh, Xu, Yang, Abdel-Hameed, Mona, Sorensen, John L., Singer, Stacy D., Chen, Guanqun
Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...
-
2020-03-01
Tian, Bo, Sun, Meijuan, Jayawardana, Kethmi, Wu, Ding, Chen, Guanqun
Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri‐ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n‐9) esterified to the sn‐2 position of phosphatidylcholine (PtdCho) catalyzed by...
-
Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...