Search
Skip to Search Results- 1Acedo, Jeella Z
- 1Bakhtiary, Alireza
- 1Cochrane, Rachel V K
- 1Engelhardt, Daniel
- 1Huang, Zedu
- 1Larade, Sean
-
Examining the structure, function and mode of action of bacteriocins from lactic acid bacteria
DownloadSpring 2010
Carnocyclin A (CclA) is a remarkably stable, potent bacteriocin produced by Carnobacterium maltaromaticum UAL307. Elucidation of the amino acid and genetic sequences revealed that CclA is a circular bacteriocin. Preliminary structural studies (dynamic light scattering, NMR, circular dichroism,...
-
Chemical Synthesis and Biological Evaluation of Unusual Sulfur-Containing Peptides and Efforts Toward Chemical Synthesis of Crocin
DownloadFall 2012
In order to minimize side reactions when preparing peptides containing a C-terminal cysteine residue, a method of protection of the carboxyl of the C-terminal cysteine as a cyclic ortho ester was developed. N-Fmoc-S-StBu cysteine cyclic ortho esters 31 (L) and 32 (D) were synthesized with...
-
Fall 2014
Paenibacillus polymyxa NRRL B-30509, Paenibacillus terrae NRRL B-30644 and P. polymyxa NRRL B-30507 were found to produce several bacteriocins and non-ribosomal peptides. All three strains produce tridecaptins, non-ribosomal lipopeptides antimicrobially active against food pathogen Campylobacter...
-
Spring 2016
Peptides have a host of beneficial physiological effects and are often considered for therapeutic application to treat disease. However, poor bioavailabilities, problems with administration, sensitivity to environmental factors, and susceptibility to proteases limit them. The work presented in...
-
Biochemical investigations of two polyketide synthase subclasses – highly reducing/non-reducing pairs, and polyketide synthase non-ribosomal peptide synthetases.
DownloadSpring 2016
Cladosporin is a polyketide metabolite that is biosynthesized by a highly reducing (HR)/non-reducing (NR) polyketide synthase (PKS) pair, wherein they catalyse head-totail condensation of acetyl and malonyl CoA units with subsequent β-keto modification. Cladosporin has recently been reported as a...
-
Biosynthetic Study of CcsA and a Polyketide Synthase with Homology to Carnitine Acyltransferase
DownloadFall 2017
Polyketides are a class of natural products with large structural and biochemical diversity. Polyketides are assembled by the polymerization of short-chain fatty acids by enzymes named polyketide synthases. Fungal iterative polyketide synthases are notable in that they contain multiple active...
-
Investigations of Fungal Highly Reducing Polyketide Synthases: The Biosyntheses of Lovastatin and Hypothemycin
DownloadFall 2017
Fungal highly reducing polyketide synthases (HR-PKSs) are large, multi-domain enzymes central to the biosynthesis of fungal polyketides. They assemble complex secondary metabolites, called polyketides, through the condensation of two-carbon acetate units and tailoring of the functional groups at...
-
Spring 2018
Antimicrobial peptides (AMPs) are small protein toxins produced by different bacterial strains to fight against closely related strains in their competitive environment. AMPs have been isolated and studied for their interesting properties, including treatment of pathogenic diseases. In this...
-
Spring 2018
Acidocin B (AcdB), a bacteriocin from Lactobacillus acidophilus M46 that was initially reported to be a linear peptide, was purified and shown to be circular based on MALDI-TOF MS and MS/MS sequencing. MS analysis further revealed that AcdB is comprised of 58 amino acid residues, instead of 59...
-
Fall 2022
Peptides can elicit a whole host of beneficial physiological effects. The market for peptide-based therapeutics is rapidly expanding due to their extreme specificity and low toxicity. Conversely, they often possess poor bioavailability, stability, pharmacokinetics and cell permeability, limiting...