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1988
Plastow, G. S., Mileham, A. J., Siggens, K. W.
A 3.8 kb male specific fragment was observed following agarose gel etectrophoresis of porcine DNA digested with Sph I. DNA was recovered from this region of a gel using DE-81 paper and ligated to Sph I digested pUC18. Plasmids carrying male specific sequences were identified by their differential...
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2001
Ciobanu, D. C., Nagy, A., Day, A. E., Plastow, G. S., Rothschild, M. F., Wales, R.
Analysis of the genetic variation of an endangered population is an important component for the success of conservation. Animals from two local Romanian pig breeds, the Mangalitsa and Bazna, were analysed for variation at a number of genetic loci using PCR-based DNA tests. Polymorphism was...
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2006
Geverink, N. A., Plastow, G. S., Foury, A., Gil, M., Gispert, M., Moisan, M. P., Hortós, M., Gort, G., Font i Furnols, M., Mormède, P.
The gene (Cbg) encoding cortisol-binding globulin (CBG) has been proposed as a candidate gene to explain genetic variation in cortisol secretion and carcass composition in pigs. The objective of this study was to evaluate the association between CBG and pork quality in 5 European breeding lines,...
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Allometric association between in vivo estimation of body composition during growth using deuterium dilution technique and chemical analysis of serial slaughtered pigs
Download2006
Looft, H., Landgraf, S., Kalm, E., Roehe, R., Plastow, G. S., Susenbeth, A., Knap, P. W.
The objective of this study was to develop accurate mathematical-statistical functions to estimate body composition of live pigs between 20 and 140 kg weight from total body water (TBWA) determined by the deuterium dilution technique. Chemical body compositions during the growth period are...
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Development of carcass cuts, organs, body tissue and chemical body composition during growth of pigs
Download2006
Landgraf, S., Roehe, R., Plastow, G. S., Kalm, E., Looft, H., Susenbeth, A., Knap, P. W.
A serial slaughter trial was carried out to examine the developmental change of physical and chemical body composition in pigs highly selected for lean content. A total of 48 pigs (17 females and 31 castrated males) were serially slaughtered and chemically analysed. Eight pigs were slaughtered at...
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2012
Xia, Y., Zhang, X., Mu, Z., Li, X., Wang, T., Qu, J., Chen, H., Li, K., Zhu, L., Wang, L., Liu, Y., Zhang, K., Guan, J., Wu, H., Jiang, A., Yin, G., Xi, D., Shuai, S., Lang, Q., Jiang, Y., Wang, Z., Luo, Z., Xiao, H., Li, Y., Huang, S., Gu, Y., Beck, S., Ma, J., Wang, H., Jing, L., Plastow, G. S., Jiang, Z., Gao, X., Chen, L., Tang, G., Wang, J., Li,R., Li, M., Mai, M., Wang, Y., Wang, X., Zhong, Z., Nie, J., Zhou, S., Yang, H., Yi, N., Li, N.
It is evident that epigenetic factors, especially DNA methylation, have essential roles in obesity development. Here, using pig as a model, we investigate the systematic association between DNA methylation and obesity. We sample eight variant adipose and two distinct skeletal muscle tissues from...
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Expansion of ruminant-specific microRNAs shapes target gene expression divergence between ruminant and non-ruminant species
Download2013
Kommadath, A., Bao, H., Meng, Y., Plastow, G. S., Stothard, P., Sun, X., Arantes, A. S., Guan, L. L.
Background Understanding how species-specific microRNAs (miRNAs) contribute to species-specific phenotypes is a central topic in biology. This study aimed to elucidate the role of ruminant-specific miRNAs in shaping mRNA expression divergence between ruminant and non-ruminant species. ...
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Candidate genes and biological pathways associated with carcass quality traits in beef cattle
Download2013
Karisa, B. K., Bruce, H. L., Plastow, G. S., Moore, S. S., Thomson, J., Wang, Z.
The objective of this study was to use the candidate gene approach to identify the genes associated with carcass quality traits in beef cattle steers at the University of Alberta Ranch at Kinsella, Canada. This approach involved identifying positional candidate genes and prioritizing them...
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MicroRNA Buffering and Altered Variance of Gene Expression in Response to Salmonella Infection
Download2014
Plastow, G. S., Bao, H., Kommadath, A., Guan, L. L., Tuggle, C. K., Stothard, P.
One potential role of miRNAs is to buffer variation in gene expression, although conflicting results have been reported. To investigate the buffering role of miRNAs in response to Salmonella infection in pigs, we sequenced miRNA and mRNA in whole blood from 15 pig samples before and after...
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Increasing gene discovery and coverage using RNA-seq of globin RNA reduced porcine blood samples
Download2014
Stothard, P., Fritz-Waters, E., Choi, I., Abrams, S. M., Kommadath, A., Hosseini, A., Plastow, G. S., Guan, L. L., Tuggle, C. K., Bao, H., Meng, Y., Lunney, J. K., Reecy, J. M., Sun, X.
Background Transcriptome analysis of porcine whole blood has several applications, which include deciphering genetic mechanisms for host responses to viral infection and vaccination. The abundance of alpha- and beta-globin transcripts in blood, however, impedes the ability to cost-effectively...