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- 2Chen, Guanqun
- 2Mietkiewska, Elzbieta
- 2Weselake, Randall J.
- 2Xu, Yang
- 1Caldo, Kristian Mark P.
- 1Chen, Yongyan
- 1Brassica napus
- 1Diacylglycerol acyltransferase
- 1Directed evolution
- 1Leaf oil production
High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure functionDownload
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to enrichment of flax seed oil with a-linolenic acidDownload
Seed oil from flax (Linum usitatissimum) is enriched in a-linolenic acid (ALA; 18:3?9cis,12cis,15cis), but the biochemical processes underlying the enrichment of flax seed oil with this polyunsaturated fatty acid are not fully elucidated. Here, a potential process involving the catalytic actions...