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- 3Weselake, Randall J.
- 2Caldo, Kristian Mark P.
- 2Chen, Guanqun
- 2Greer, Michael S.
- 2Pan, Xue
- 1 Lemieux, M. Joanne
Structural and Biochemical Changes in Loblolly Pine (Pinus taeda L.) Seeds during Germination and Early Seedling Growth. II. Storage Triacylglycerols and CarbohydratesDownload
Abstract: Triacylglycerols (TAGs) comprised 59% of the total storage reserve in mature loblolly pine (Pinus taeda L.) seeds; 80% of these TAGs were stored in the megagametophyte. The TAG breakdown in the seedling was initiated before radicle emergence (during germination), while in the...
A 10 kDa acyl-CoA binding protein (ACBP) from Brassica napus enhances acyl exchange between acyl-CoA and phosphatidylcholineDownload
The gene encoding a 10-kDa acyl-CoA-binding protein (ACBP) from Brassica napus wasover-expressed in developing seeds of Arabidopsis thaliana. Biochemical analysis of T2andT3A. thaliana seeds revealed a signiﬁcant increase in polyunsaturated fatty acids (FAs)(18:2cisD9,12and 18:3cisD9,12,15) at...
Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinoleninDownload
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferaseDownload
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1Download
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 22.214.171.124). Improving DGAT...