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  • Evaluation of the Protection Induced by a Monotherapy of Anti-LFA-1 Monoclonal Antibody and Co-transplantation of Neonatal Porcine Islets with Sertoli Cells
  • Bayrack, Kevin R
  • English
  • Xenotransplantation
    Neonatal Porcine Islets
    Sertoli Cells
  • Dec 19, 2011 4:11 PM
  • Thesis
  • English
  • Adobe PDF
  • 2433747 bytes
  • Two major barriers to islet transplantation are the need for an unlimited source of donor tissue and a safer method of immunosuppression. These may be overcome by xenotransplantation of neonatal porcine islets (NPI) along with combined co-transplantation of neonatal porcine Sertoli cells (SC) and transient use of anti-LFA-1 monoclonal antibody (mAb). In this study we aimed to identify potential mechanisms responsible for prolonged NPI islet xenograft survival with our combination therapy. Our data demonstrates that the combination of anti-LFA-1 mAb therapy along with the co-transplantation of SC is indeed highly efficacious in preventing NPI xenograft rejection as 20/27 treated mice achieved and maintained long-term graft survival. Although it appears that T regulatory cells are not solely responsible for maintaining long-term xenograft protection, they are likely important in establishing a TH2 cell phenotype and sharing a role with secreted SC products, such as serpina3n, in prolonging NPI xenograft protection.
  • Ramji QA, Bayrack K, Arefanian H, Marcet-Palacios M, Bleackley RC, Rajotte RV, Rayat GR. Protection of porcine islet xenografts in mice using sertoli cells and monoclonal antibodies. Transplantation 92(12): 1309-1315 (2011).
  • Master's
  • Master of Science
  • Department of Surgery
  • Experimental Surgery
  • Spring 2012
  • Rayat, Gina (Surgery)
    Rajotte, Raymond (Surgery)
  • Rayat, Gina (Surgery)
    Rajotte, Raymond (Surgery)
    Bleackley, R Chris (Biochemistry)