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  • http://hdl.handle.net/10402/era.24915
  • Identification of dehydration tolerance genes in triticale (x Triticosecale Wittm.) seedlings
  • Badea, Cosmin
  • English
  • dehydration stress
    triticale
    plant science
  • Jan 4, 2012 3:43 PM
  • Thesis
  • English
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  • Dehydration stress triggers many changes in plant gene expression. Triticale (x Triticosecale Wittm.) sequencing using next generation sequencing allowed identification of 107,000 transcripts of which 4,467 were differentially regulated ≥2-fold. Abscisic acid co-receptors, pyrabactin and protein phosphatases 2C were highly regulated and identified for the first time in dehydration stress. Many ABA-independent genes such as DREB/CBFs family were down-regulated or non-differentially expressed compared to non-stressed plants. This suggested that the solid polyethylene glycol system successfully led to identification of dehydration stress-specific genes from other stresses. Only 5% from the 87% annotated differentially regulated transcripts showed homology to previously identified dehydration responsive genes in wheat, maize, barley or Arabidopsis. Thus, this study revealed a larger number of novel genes potentially involved in dehydration stress, many of which were highly differentially regulated. Identification of differentially expressed genes and their functions during dehydration stress is essential towards improvement of dehydration tolerance crops.
  • Master's
  • Master of Science
  • Department of Agricultural, Food, and Nutritional Science
  • Plant Science
  • Spring 2012
  • Weselake, Randall (AFNS), Laroche, André (AAFC)
  • Michael Deyholos (Biological Science)
    Kav, Nataraj (AFNS)
    Weselake, Randall (AFNS)
    Laroche, André (AAFC)
    King, Jane (AFNS) Chair