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  • http://hdl.handle.net/10402/era.29411
  • Advanced Materials and Detection Methods in Ultrathin-Layer Chromatography
  • Oko, Anthony J
  • English
  • Chromatography
    GLAD
    UTLC
  • Nov 21, 2012 2:55 PM
  • Thesis
  • English
  • Adobe PDF
  • 3632358 bytes
  • The performance of highly anisotropic, nanostructured, thin-film ultrathin-layer chromatography (UTLC) media with porosity and architecture engineered using the glancing-angle deposition (GLAD) process is investigated. Anisotropic structures resembling nanoblades are fabricated on the plates, producing channel-like features that partially decouple analyte migration from development direction, and offering new separation behaviours. These GLAD UTLC plates provide channel features that reduce transverse spot broadening while providing the wide pores required for rapid migration and high separation performance with plate numbers approaching 800. The rapid separations typical of these miniaturized plates call for new alternative detection techniques. I have designed, implemented and characterized a measurement system which records UTLC separations in full colour with 32 µm spatial resolution and 33 ms temporal resolution. My code analyzes multiple tracks per plate, filters analyte spots by colour, and automatically generates time-resolved figures of merit. Both absorption and transmission detection modes are examined, each of which offers their own advantages.
  • Oko, A. J., Jim, S. R., Taschuk, M. T. & Brett, M. J. Analyte migration in anisotropic nanostructured ultrathin-layer chromatography media. Journal of chromatography. A 1218, 2661–7 (2011).
    Oko, A. J., Jim, S. R., Taschuk, M. T. & Brett, M. J. Time resolved chromatograms in ultra-thin layer chromatography. Journal of chromatography. A 1249, 226–32 (2012).
  • Master's
  • Master of Science
  • Department of Electrical and Computer Engineering
  • Microsystems and Nanodevices
  • Spring 2013
  • Brett, Michael (Electrical and Computer Engineering)
  • Brett, Michael (Electrical and Computer Engineering)
    Sit, Jeremy (Electrical and Computer Engineering)
    Chen, Weixing (Chemical and Materials Engineering)