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  • http://hdl.handle.net/10402/era.27496
  • Population genomics of North American grey wolves (Canis lupus)
  • Knowles, James
  • English
  • wolves
    genomics
    SNPs
    structure
    selection
    population
    microarray
  • Jul 26, 2010 9:38 PM
  • Thesis
  • English
  • Adobe PDF
  • 2153751 bytes
  • Previous studies of the grey wolf (Canis lupus) using microsatellites have showed strong population structure despite the high mobility of individuals. I re-assessed the structure of North American grey wolves by genotyping 132 wolves at a genome-wide set of >26 000 single nucleotide polymorphisms (SNPs), and found less population structure, a strong pattern of isolation by distance, and determined that gene flow between subpopulations relates to prey specialization. To assess how accurately smaller data sets assign individuals, I analyzed sub-sets of SNPs and found that small marker sets varied greatly in estimates of subpopulation assignment, and showed high discordance with assignments determined when using all 26k markers. Finally, using a genome scan to detect natural selection I identified SNPs in three genes that may have undergone directional selection, contain variation with observed phenotypic consequences in other mammal species and may be related to adaptation in grey wolves.
  • Master's
  • Master of Science
  • Department of Biological Sciences
  • Fall 2010
  • Coltman, David (Biological Sciences)
  • Hall, Jocelyn (Biological Sciences)
    Moore, Stephen (Agricultural, Food and Nutritional Science)