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  • http://hdl.handle.net/10402/era.24977
  • Recombinant expression of plant diacylglycerol acyltransferases from tissues that accumulate saturated fatty acids
  • Zhang, Ying
  • English
  • sea buckthorn pulp, cocoa bean, diacylglycerol acyltransferases, triacylglycerol, cloning
  • Jan 6, 2012 12:29 PM
  • Thesis
  • English
  • Adobe PDF
  • 2810410 bytes
  • Vegetable oils enriched in saturated fatty acids (SFAs) could provide solid-fat functionality and decrease our reliance on hydrogenated oils. This thesis focuses on the characterization of enzymes that can preferentially incorporate SFAs into triacylglycerol (TAG). Acyl-CoA:diacylglycerol acyltransferase (DGAT) catalyzes the final reaction in acyl-CoA-dependent TAG biosynthesis, and may have a substantial effect on the flow of carbon into TAG. DGAT cDNAs encoding type-1 or 2 enzymes were isolated from sea buckthorn (Hippophae rhamnoides) pulp and cocoa (Theobroma cacao) beans, both of which contain oils enriched in SFAs. Expression of the HrDGATs or TcDGATs resulted in different ratios of palmitic to stearic acid in the TAG of yeast mutant strain deficient in TAG synthesis. Furthermore, expression of HrDGAT1 in developing seeds of the Arabidopsis DGAT1 knockout mutant restored TAG accumulation and altered the fatty acid composition. These results suggest that HrDGATs or TcDGATs could potentially be used to increase the SFA content of vegetable oils.
  • Master's
  • Master of Science
  • Department of Agricultural, Food, and Nutritional Science
  • Plant Science
  • Spring 2012
  • Weselake, Randall J. (Agricultural, Food and Nutritional Science)
  • Weselake, Randall J. (Agricultural, Food and Nutritional Science)
    Gaenzle, Michael (Agricultural, Food and Nutritional Science)
    Scarpella, Enrico (Biological Science)