- The study of claudins in a model system of the proximal tubule
- Borovac, Jelena
- Nov 30, 2012 12:19 PM
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- The proximal tubule (PT) is a major site of paracellular transport. Research in this area is undermined by the lack of a model system that is representative of PT electrophysiology. We examined opossum kidney (OK) cell characteristics and found a striking resemblance to the PT (transepithelial resistance = 11.7 Ω cm2 and the permeability ratio PNa+/PCl- = 1.08). Furthermore, OK cells express a number of claudins (claudin-4 > -1 > -6 > -20 > -9 > -12 > -11 > -15, in order of the amount of expression), which are known to regulate paracellular transport. We over-expressed claudin-2 and found that it forms a non-selective pore at the tight junction of OK cells. Over-expression of claudin-4, on the other hand, led to the formation of a non-selective barrier. However, the expression of these isoforms caused significant perturbations in expression of endogenous claudins leading us to conclude that claudin expression is strongly interdependent.
- Borovac J, Barker RS, Rievaj J, Rasmussen A, Pan W, Wevrick R, and Alexander RT. Claudin-4 forms a paracellular barrier, revealing the interdependence of claudin expression in the loose epithelial cell culture model, Opossum Kidney Cells. Am J Physiol Cell Physiol 2012. Oct 17. [Epub ahead of print]
- Master of Science
- Department of Physiology
- Spring 2013
- Alexander, RT
Wevrick, Rachel (Medical Genetics)
Theses and Dissertations Spring 2009 to present
Department of Physiology
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