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  • http://hdl.handle.net/10402/era.29432
  • The study of claudins in a model system of the proximal tubule
  • Borovac, Jelena
  • English
  • Proximal tubule
    Claudin
    Paracellular
  • Nov 30, 2012 12:19 PM
  • Thesis
  • English
  • Adobe PDF
  • 3835487 bytes
  • The proximal tubule (PT) is a major site of paracellular transport. Research in this area is undermined by the lack of a model system that is representative of PT electrophysiology. We examined opossum kidney (OK) cell characteristics and found a striking resemblance to the PT (transepithelial resistance = 11.7 Ω cm2 and the permeability ratio PNa+/PCl- = 1.08). Furthermore, OK cells express a number of claudins (claudin-4 > -1 > -6 > -20 > -9 > -12 > -11 > -15, in order of the amount of expression), which are known to regulate paracellular transport. We over-expressed claudin-2 and found that it forms a non-selective pore at the tight junction of OK cells. Over-expression of claudin-4, on the other hand, led to the formation of a non-selective barrier. However, the expression of these isoforms caused significant perturbations in expression of endogenous claudins leading us to conclude that claudin expression is strongly interdependent.
  • Borovac J, Barker RS, Rievaj J, Rasmussen A, Pan W, Wevrick R, and Alexander RT. Claudin-4 forms a paracellular barrier, revealing the interdependence of claudin expression in the loose epithelial cell culture model, Opossum Kidney Cells. Am J Physiol Cell Physiol 2012. Oct 17. [Epub ahead of print]
  • Master's
  • Master of Science
  • Department of Physiology
  • Spring 2013
  • Alexander, RT
  • Wevrick, Rachel (Medical Genetics)
    Chen, Xing-Zhen
    Cordat, Emmanuelle