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  • http://hdl.handle.net/10402/era.17197
  • Engineering nitrogen use efficiency with alanine aminotransferase.
  • Good, A.G.
    Johnson, S.J.
    DePauw, M.
    Carroll, R.T.
    Savidov, N.
    Vidmar, J.J.
    Lu, Z.
    Taylor, G.T.
    Stroeher,V.
  • agricultural productivity
    nitrogen
    crops and nitrogen
    fertilizers
    barley
    transgenic plants
    crop yields
    plant genetic engineering
  • Journal Article (Published)
  • English
  • Nitrogen (N) is the most important factor limiting crop productivity worldwide. The ability of plants to acquire N from applied fertilizers is one of the critical steps limiting the efficient use of nitrogen. To improve N use efficiency, genetically modified plants that overexpress alanine aminotransferase (AlaAT) were engineered by introducing a barley AlaAT cDNA driven by a canola root specific promoter (btg26). Compared with wild-type canola, transgenic plants had increased biomass and seed yield both in the laboratory and field under low N conditions, whereas no differences were observed under high N.The transgenics also had increased nitrate influx. These changes resulted in a 40% decrease in the amount of applied nitrogen fertilizer required under field conditions to achieve yields equivalent to wild-type plants
  • Good, A.G., S.J. Johnson, M. DePauw, R.T. Carroll, N. Savidov, J.J. Vidmar, Z. Lu, G.T. Taylor and V. Stroeher. (2007) Engineering nitrogen use efficiency with alanine aminotransferase. Can J. Bot. 85:252-262.