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  • http://hdl.handle.net/10402/era.25516
  • The Role of Rho GTPases, Rac1 and Rac2, in Mast Cell Exocytosis
  • Baier, Alicia
  • English
  • Rac1 and Rac2 GTPases
    mast cell
    exocytosis
  • Feb 27, 2012 3:23 PM
  • Thesis
  • English
  • Adobe PDF
  • 3098619 bytes
  • Mast cells are tissue-resident immune cells that undergo exocytosis upon activation, releasing potent immunoregulatory molecules that initiate inflammatory responses. Here, I investigated the role of the hematopoietic specific Rho GTPase, Rac2, and the ubiquitous, Rac1, on mast cell exocytosis. Our hypothesis is that Fc(epsilon)RI-mediated mast cell exocytosis is differentially regulated by Rac1 and Rac2 GTPases. Three experimental systems were used to investigate their roles in mast cell function; Rac2 knockout mice, Rac1 and Rac2 siRNA knockdown, and treatment with the Rac inhibitor, EHT-1864. My results suggest that Rac1 and Rac2 regulate multiple stages of Fc(epsilon)RI-mediated response in mast cells. I found that calcium flux and exocytosis were Rac1 and Rac2-dependent processes. My data suggests that Rac1 and Rac2 regulate exocytosis through a convergent pathway but regulate calcium flux through alternate pathways. Moreover, I found that Rac2 does not regulate actin remodelling, suggesting this may be a Rac1-mediated process.
  • Master's
  • Master of Science
  • Department of Cell Biology
  • Spring 2012
  • Eitzen, Gary (Cell Biology)
  • LaPointe, Paul (Cell Biology)
    Vliagoftis, Harissios (Medicine)
    Lacy, Paige (Medicine)