Validation of Reagent-free GC Lipid Derivatization Method Using an Enzymatic Microreactor

• Author / Creator

  Zhang, Yiran

• Derivatization of fatty acids to produce volatile methyl or ethyl esters (FAME or FAEE) prior to GC analysis is an indispensable procedure in lipid analysis. A lipase immobilized porous polymer monolith microreactor was developed and shown to achieve online and quantitative conversion of triglycerides to FAEE. When in use, a low flow of oil in ethanol is passed through the 15cm long microreactor. Fully conversion of oil into FAEE will be achieved during the passage of the solution through the enzymatic microreactor, so that the products can be collected for direct GC analysis. Here we describe an optimization and a validation of the first generation microreactor for reagent-free derivatization of vegetable oil samples with varied fatty acid distributions. Results will be presented in order to demonstrate (i) artifact-free quantitative FAEE formation giving equivalent overall accuracy compared to AOCS method Ce 1k-09 for FAME; (ii) microreactor intermediate precision and reusability; (iii) achieve conversion in a shorter time. These attributes are required for a future automated system.

• Subjects / Keywords

  • GC
  • Lipid derivatization
  • Microreactor

• Graduation date

  Fall 2014

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3P84442R

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.