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Comparative Genomics of <i>Vibrio metoecus</i> with Its Close Relative <i>Vibrio cholerae</i> Reveals Its Pathogenic Potential

  • Author(s) / Creator(s)
  • Presented at the "Genomics: The Power and the Promise" conference in Ottawa, Ontario on November 24-26, 2014.<br><br>Abstract published on: Genome (2014), 57(7):377-378<br><br><i>Vibrio metoecus</i> is a recently discovered species that is closely related to <i>Vibrio cholerae</i>, the causative agent of the potent disease cholera. It has been co-isolated with <i>V. cholerae</i> in coastal waters and found in clinical specimens in the United States. Our aim is to understand the pathogenic potential of <i>V. metoecus</i> and the extent of genetic interaction with <i>V. cholerae</i>. By comparison of 48 genomes of <i>V. metoecus</i> (10 genomes) and <i>V. cholerae</i> (38 genomes) using various bioinformatics tools, both species display 85-86% average nucleotide identity (ANI), which indicate very distinct species (>95% ANI for the same species). <i>V. metoecus</i> strains do not encode the major virulence factors of pandemic <i>V. cholerae</i> strains, the CTX and TCP gene clusters. However, it encodes other virulence factors and genomic islands, such as multiple hemolysin and neuraminidase genes, and regions of the <i>Vibrio</i> seventh pandemic islands (VSP) I and II and <i>Vibrio</i> pathogenicity island II. Both VSP islands are also present in pathogenic <i>V. cholerae</i> strains of the seventh pandemic. Interestingly, the superintegron, a region of the genome that is able to acquire genes and is the main cause of multiple antibiotic resistance in bacteria, shows higher conservation in gene content between <i>V. metoecus</i> and <i>V. cholerae</i>, isolated from the same environment, than any other region of their genomes, despite having the least conservation in gene order. Additionally, there is evidence of recombination inside the superintegron region and between housekeeping genes. Results suggest recent horizontal gene transfer of these mobile genetic elements between species, raising the concern that more virulence genes could cross the species barrier from <i>V. cholerae</i> to <i>V. metoecus</i>, facilitating the rise of this novel emerging pathogen, which is in constant interaction with <i>V. cholerae</i>.

  • Date created
    2014-11-23
  • Subjects / Keywords
  • Type of Item
    Conference Poster
  • DOI
    https://doi.org/10.7939/R3DJ58K2W
  • License
    Attribution-NonCommercial-NoDerivatives 3.0 International
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  • Citation for previous publication
    • <b>Orata FD</b>, Kirchberger PC, Boucher Y. (2014) Comparative genomics of <i>Vibrio metoecus</i> with its close relative <i>Vibrio cholerae</i> reveals its pathogenic potential. <i>Genome</i>, 57(7):377-378.