Characterization of novel galactosylated chitin-oligosaccharides and chitosan-oligosaccharides

• Author(s) / Creator(s)

  • Black, Brenna A.
  • Yan, Yalu
  • Galle, Sandra
  • Hu, Ying
  • Curtis, Jonathan M.
  • Gänzle, Michael G.

• This study determined whether chitin-oligosaccharides and chitosan-oligosaccharides (COS) are suitable acceptor carbohydrates for the LacLM-type β-galactosidase of Lactobacillus plantarum. Enzymatic reactions were carried out with lactose as galactosyl-donor and chitinbiose (N,N′-diacetyl chitobiose), chitintriose (N,N′N″-triacetyl chitotriose) or a highly deacetylated COS preparation with a degree of polymerization ranging from 2 to 4 as galactosyl acceptors. Transgalactosylated oligosaccharides were identified by liquid chromatography-mass spectrometry (LC-MS/MS). LC-MS/MS analysis demonstrated formation of mono- and digalactosylated chitinbiose, mono-galactosylated chitintriose, and mono-, di-, and trigalactosylated COS. β-(1→4)-Linkages were formed during the galactosylation of chitinbiose and chitintriose. In conclusion, the conversion of lactose with β-Gal from Lb. plantarum and chitin-oligosaccharides or COS as glycosyl acceptors allows the production of novel oligosaccharides.

• Date created

  2014-12-01

• Subjects / Keywords

  • Lactobacillus plantarum

• Type of Item

  Article (Draft / Submitted)

• DOI

  https://doi.org/10.7939/r3-e9y7-x632

• License

  Attribution-NonCommercial-NoDerivatives 4.0 International