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Permanent link (DOI): https://doi.org/10.7939/R3GM8205P

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Phylogenomics of the Choristoneura fumiferana species complex (Lepidoptera: Tortricidae) Open Access

Descriptions

Other title
Subject/Keyword
jack pine budworm
Choristoneura pinus
Choristoneura fumiferana
phylogenetics
evolution
Lepidoptera
spruce budworm
systematics
Genotyping by Sequencing
Phylogenomics
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Bird, Heather M
Supervisor and department
Sperling, Felix (Biological Sciences)
Examining committee member and department
Stothard, Paul (Agricultural, Food and Nutritional Science)
Gallin, Warren (Biological Sciences)
Hall, Jocelyn (Biological Sciences)
Department
Department of Biological Sciences
Specialization
Systematics and Evolution
Date accepted
2013-09-24T10:16:50Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
The phylogenetic relationships of the destructive spruce budworm group of forest pests (Choristoneura fumiferana species complex) have previously been explored using allozymes, microsatellites, mitochondrial genes and a nuclear gene, but remain poorly resolved with conflicting topologies. I used mass sampling of single nucleotide polymorphisms (SNPs) across their genome in a genotyping-by-sequencing approach. Over 100 000 SNPs with greater than 75% coverage in 99 specimens (ApeKI restriction enzyme digest) or 144 specimens (PstI-MspI digest) resolved C. fumiferana, C. carnana, C. retiniana, and C. pinus as strongly-supported monophyletic species. The most distinct species, C. pinus, yielded 945 autapomorphic SNPs, and was definitively placed as basal to the whole species complex, contrasting with previous mtDNA results. The functions of genes homologous to the sequence surrounding the diagnostic C. pinus SNPs included detoxification, morphological differences, flight, and sensory perception, providing insights into the genetic basis of species differences.
Language
English
DOI
doi:10.7939/R3GM8205P
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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