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Permanent link (DOI): https://doi.org/10.7939/R31K9F

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Rescue of Kidney Anion Exchanger 1 Trafficking Mutants Open Access

Descriptions

Other title
Subject/Keyword
Trafficking
Anion Exchanger
Degradation
Rescue
Chemicals
Small molecules
Kidney
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Chu, Carmen Y.S.
Supervisor and department
Cordat, Emmanuelle (Physiology)
Examining committee member and department
Melancon, Paul (Cell Biology)
Cheeseman, Chris (Physiology)
Department
Department of Physiology
Specialization

Date accepted
2012-08-31T12:11:38Z
Graduation date
2012-11
Degree
Master of Science
Degree level
Master's
Abstract
The kidney anion exchanger 1 (kAE1) is crucial in the regulation of physiological pH by facilitating Cl-/HCO3- exchange. Inability to do so results in distal renal tubular acidosis (dRTA), which is more often due to mutations leading to mis-localization of kAE1, rather than complete abolishment of its functional activity. The purpose of this thesis is to understand the trafficking, turnover, and rescue of wildtype (WT) and trafficking mutants of the kAE1. Chemical chaperones and low temperature treatments were studied for their ability to rescue trafficking of G701D, C479W and R589H kAE1 mutants. G701D kAE1 was rescued by dimethyl sulfoxide (DMSO) and is functional at the membrane. The turnover of all kAE1 mutants studied was dependent on the proteasomal pathway, while G701D and WT kAE1 are also degraded via the lysosomal pathway. These studies provide essential information for developing rescue methods at the molecular level, targeting the source of the disease rather than the symptoms.
Language
English
DOI
doi:10.7939/R31K9F
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File format: pdf (Portable Document Format)
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File size: 13391003
Last modified: 2015:10:18 01:35:04-06:00
Filename: thesis v4 corrected.pdf
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Status message: File header gives version as 1.4, but catalog dictionary gives version as 1.3
File title: Microsoft Word - thesis v4 corrected.docx
File author: Emmanuelle Cordat
Page count: 128
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