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Permanent link (DOI): https://doi.org/10.7939/R3M61BX42

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Application of antimicrobials for the elimination of Escherichia coli and Listeria monocytogenes in brine injected beef Open Access

Descriptions

Other title
Subject/Keyword
antimicrobial
brine injection
minimum bactericidal concentration
beef
Listeria monocytogenes
heat resistance
Escherichia coli
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Ward, Patrick J
Supervisor and department
Nattress, Frances (Agricultural, Food and Nutritional Science)
Gaenzle, Michael (Agricultural, Food and Nutritional Science)
McMullen, Lynn (Agricultural, Food and Nutritional Science)
Examining committee member and department
Nattress, Frances (Agricultural, Food and Nutritional Science)
McMullen, Lynn (Agricultural, Food and Nutritional Science)
Betti, Mirko (Agricultural, Food and Nutritional Science)
Gaenzle, Michael (Agricultural, Food and Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Food Science and Technology
Date accepted
2013-09-30T14:19:47Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
The application of antimicrobials to a brine-injected raw beef roast for the elimination/inhibition of heat resistant Escherichia coli and Listeria monocytogenes was investigated. The choice of antimicrobials for use in brine injected beef was based on minimum bactericidal concentration in brine solutions. Charsol® and MicocinX™, were added to the brine solution individually and in combination to evaluate possible synergy between the antimicrobials. In the brine solution, numbers of E. coli were not reduced by either antimicrobial; however, L. monocytogenes was reduced by more than 2 log CFU/mL in the presence of either antimicrobial, and no synergistic effect was detected. For the brine-injected beef, neither antimicrobial had any effect on numbers of E. coli, and the counts remained the same during 7 days of storage at 7°C. L. monocytogenes was unaffected by the Charsol®; however, counts were reduced when MicocinX™ was present and growth was inhibited during 4 days of storage.
Language
English
DOI
doi:10.7939/R3M61BX42
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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