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Permanent link (DOI): https://doi.org/10.7939/R3SQ8QQ3T

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Effects of bulky polycyclic aromatic hydrocarbon adducts on DNA replication by exonuclease-deficient T7 and T4 DNA polymerases Open Access

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Author or creator
Keohavong, P.
Shukla, R.
Melacrinos, A.
Day, B. W.
Reha-Krantz, L.
Additional contributors
Subject/Keyword
Invitro
Tumorigenicity
Fluoranthene
Mutations
In-vitro replication
Benzo-A-Pyrene diol epoxide
Cyclopenta-CD-Pyrene
Mouse lung
HPRT gene
Lung adenoma bioassay
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Abstract: In vitro DNA replication by exonuclease-deficient T7 DNA polymerase (Sequenase) and an exonuclease deficient T4 DNA polymerase was examined on a 244-nucleotide DNA template treated with three electrophilic polycyclic aromatic hydrocarbon (PAH) metabolites: racemic trans-7,8-dihydroxy-anti-9,10-epoxy-7,8, tetrahydrobenzo[a]pyrene (BaPDE), trans-2,3-dihydroxy-anti-1,10b-epoxy-10b,1,2,3-tetrahydrofluoranthene (FADE), or 3,4-epoxy-3,4-dihydrocyclopenta[cd]pyrene (CPPE), The DNA replication terminated opposite template guanines and, to a lesser extent, at template adenines, as expected, as purines were modified preferentially by the chemical treatments. Analysis of the products synthesized on the damaged templates indicated that bypass replication by Sequenase proceeded in three steps: (1) replication first terminated one base 3' to each adduct; (2) a nucleotide was then incorporated opposite the PAM-modified base; and (3) replication continued at some sites to give full bypass of the lesions. The rate of lesion bypass was affected by the type of chemical adduct, the sequence context of the adduct, and the concentration of deoxynucleoside triphosphates, Short DNA repeats appeared to facilitate translesion replication.
Date created
1998
DOI
doi:10.7939/R3SQ8QQ3T
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This is a copy of an article published in the DNA and Cell Biology © 1998, copyright Mary Ann Liebert, Inc.; DNA and Cell Biology is available online at: http://online.liebertpub.com. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
Citation for previous publication
Keohavong, P., Shukla, R., Melacrinos, A., Day, B. W., & Reha-Krantz, L. (1998). Effects of bulky polycyclic aromatic hydrocarbon adducts on DNA replication by exonuclease-deficient T7 and T4 DNA polymerases. DNA and Cell Biology, 17(6), 541-549. DOI: 10.1089/dna.1998.17.541.
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File title: Effects of Bulky Polycyclic Aromatic Hydrocarbon Adducts on DNA Replication by Exonuclease-Deficient T7 and T4 DNA Polymerases
File author: PHOUTHONE KEOHAVONG, RAJIV SHUKLA, ALEA MELACRINOS, BILLY W. DAY, and LINDA REHA-KRANTZ
Page count: 10
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