Download the full-sized PDF of Advanced Materials and Detection Methods in Ultrathin-Layer ChromatographyDownload the full-sized PDF


Download  |  Analytics

Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Faculty of Graduate Studies and Research


This file is in the following collections:

Theses and Dissertations

Advanced Materials and Detection Methods in Ultrathin-Layer Chromatography Open Access


Other title
Type of item
Degree grantor
University of Alberta
Author or creator
Oko, Anthony J
Supervisor and department
Brett, Michael (Electrical and Computer Engineering)
Examining committee member and department
Sit, Jeremy (Electrical and Computer Engineering)
Chen, Weixing (Chemical and Materials Engineering)
Brett, Michael (Electrical and Computer Engineering)
Department of Electrical and Computer Engineering
Microsystems and Nanodevices
Date accepted
Graduation date
Master of Science
Degree level
The performance of highly anisotropic, nanostructured, thin-film ultrathin-layer chromatography (UTLC) media with porosity and architecture engineered using the glancing-angle deposition (GLAD) process is investigated. Anisotropic structures resembling nanoblades are fabricated on the plates, producing channel-like features that partially decouple analyte migration from development direction, and offering new separation behaviours. These GLAD UTLC plates provide channel features that reduce transverse spot broadening while providing the wide pores required for rapid migration and high separation performance with plate numbers approaching 800. The rapid separations typical of these miniaturized plates call for new alternative detection techniques. I have designed, implemented and characterized a measurement system which records UTLC separations in full colour with 32 µm spatial resolution and 33 ms temporal resolution. My code analyzes multiple tracks per plate, filters analyte spots by colour, and automatically generates time-resolved figures of merit. Both absorption and transmission detection modes are examined, each of which offers their own advantages.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Oko, A. J., Jim, S. R., Taschuk, M. T. & Brett, M. J. Analyte migration in anisotropic nanostructured ultrathin-layer chromatography media. Journal of chromatography. A 1218, 2661–7 (2011).Oko, A. J., Jim, S. R., Taschuk, M. T. & Brett, M. J. Time resolved chromatograms in ultra-thin layer chromatography. Journal of chromatography. A 1249, 226–32 (2012).

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 3632358
Last modified: 2015:10:12 20:13:43-06:00
Filename: Oko_Anthony_Fall 2012.pdf
Original checksum: 12d497185144b62cacf9a97376607f19
Well formed: true
Valid: true
Page count: 152
File language: en-CA
Activity of users you follow
User Activity Date