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Advanced Materials and Detection Methods in Ultrathin-Layer Chromatography Open Access


Other title
Type of item
Degree grantor
University of Alberta
Author or creator
Oko, Anthony J
Supervisor and department
Brett, Michael (Electrical and Computer Engineering)
Examining committee member and department
Sit, Jeremy (Electrical and Computer Engineering)
Chen, Weixing (Chemical and Materials Engineering)
Brett, Michael (Electrical and Computer Engineering)
Department of Electrical and Computer Engineering
Microsystems and Nanodevices
Date accepted
Graduation date
Master of Science
Degree level
The performance of highly anisotropic, nanostructured, thin-film ultrathin-layer chromatography (UTLC) media with porosity and architecture engineered using the glancing-angle deposition (GLAD) process is investigated. Anisotropic structures resembling nanoblades are fabricated on the plates, producing channel-like features that partially decouple analyte migration from development direction, and offering new separation behaviours. These GLAD UTLC plates provide channel features that reduce transverse spot broadening while providing the wide pores required for rapid migration and high separation performance with plate numbers approaching 800. The rapid separations typical of these miniaturized plates call for new alternative detection techniques. I have designed, implemented and characterized a measurement system which records UTLC separations in full colour with 32 µm spatial resolution and 33 ms temporal resolution. My code analyzes multiple tracks per plate, filters analyte spots by colour, and automatically generates time-resolved figures of merit. Both absorption and transmission detection modes are examined, each of which offers their own advantages.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Oko, A. J., Jim, S. R., Taschuk, M. T. & Brett, M. J. Analyte migration in anisotropic nanostructured ultrathin-layer chromatography media. Journal of chromatography. A 1218, 2661–7 (2011).Oko, A. J., Jim, S. R., Taschuk, M. T. & Brett, M. J. Time resolved chromatograms in ultra-thin layer chromatography. Journal of chromatography. A 1249, 226–32 (2012).

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