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Permanent link (DOI): https://doi.org/10.7939/R3JS8S

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Assessments of novel transgenic germplasm and treatment with the phytohormone cytokinin for reducing feeding damage by flea beetles, Phyllotreta spp. (Coleoptera: Chrysomelidae) in canola Open Access

Descriptions

Other title
Subject/Keyword
Phyllotreta
PR10.1
Transgenic
PR10.4
Striolata
Canola
Resistance
Cruciferae
Feeding
Cytokinin
ABR17
Damage
Helix
Flea
Beetle
Thiamethoxam
6-benzylaminopurine
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Sultani, Ali Sina
Supervisor and department
Dr. Nat Kav, Department of Agricultural, Food and Nutritional Sciences
Dr. Lloyd Dosdall, Department of Agricultural, Food and Nutritional Sciences
Examining committee member and department
Dr. Michael Deyholos, Department of Biological Sciences
Department
Department of Agricultural, Food, and Nutritional Science
Specialization

Date accepted
2011-09-30T18:27:47Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Flea beetles [Phyllotreta cruciferae (Goeze) and Phyllotreta striolata (Fabricius)] (Coleoptera: Chrysomelidae) pose significant threats to early canola seedling establishment. The resistance potential of transgenic Brassica napus expressing cDNA encoding pea PR10.1 and 10.4 (ABR17) in Westar and DH19 backgrounds, respectively, was investigated. In addition, the effects of treatments with different concentrations of the cytokinin 6-benzylaminopurine (BAP) and with Helix® (thiamethoxam insecticide) on flea beetle tolerance were investigated. Our results indicate that transgenic lines expressing PR10.1 and PR10.4 suffered the least damage from P. cruciferae when compared with Helix®-treated doubled haploid or Westar genotypes, while cytokinin treatments conferred some tolerance. P. striolata experiments demonstrated that insecticide treatments were most effective for reducing damage. Helix® treatments displayed the most significant beetle mortality while neither PR10.1, PR10.4 nor cytokinin treatments demonstrated insecticidal activity. Proteomic analysis revealed significant increases in abundance of some proteins including protease inhibitors in ABR17 seedlings damaged by herbivory.
Language
English
DOI
doi:10.7939/R3JS8S
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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