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Permanent link (DOI): https://doi.org/10.7939/R3QG9M

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Structure and function analysis of the Merlin Sip1 complex Open Access

Descriptions

Other title
Subject/Keyword
Sip1
Merlin
NF2
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Leung, Albert Chun Cheung
Supervisor and department
Hughes, Sarah C (Medical Genetics, Cell Biology)
Examining committee member and department
Lapointe, Paul (Cell Biology)
Berry, Fred (Medical Genetics, Surgery)
Eitzen, Gary (Cell Biology)
Department
Medical Sciences - Medical Genetics
Specialization

Date accepted
2011-10-02T16:15:06Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Mutations in the NF2 gene lead to the disease Neurofibromatosis Type II which is characterized by the formation of multiple tumours in the central nervous system. The NF2 gene encodes the protein Merlin. Merlin interacts with a number of different proteins and these interactions may be important for Merlin tumour suppressor function. Amongst these proteins, EBP50 has been identified as a candidate scaffold protein. In this thesis, I identified a potential novel binding site in Drosophila Merlin to the Drosophila EBP50 homolog, Sip1. I determined that this potential novel binding site is both necessary and sufficient for binding in vitro. In addition, a conserved potential novel phosphorylation site is also identified in this region and may be involved in the regulation of the Merlin Sip1 interaction. These results suggest that a regulatory protein complex may be involved in Merlin tumour suppressor activity.
Language
English
DOI
doi:10.7939/R3QG9M
Rights
License granted by Albert Leung (acleung@ualberta.ca) on 2011-09-30T20:38:01Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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