ERA

Download the full-sized PDF of Initial adhesion of EPS producing bacteria Burkholderia cepacia – the impact of cranberry juiceDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3TH40

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Initial adhesion of EPS producing bacteria Burkholderia cepacia – the impact of cranberry juice Open Access

Descriptions

Other title
Subject/Keyword
EPS
surface free energy
cranberry juice
bacterial adhesion
interaction forces
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Yang, Xuejiao
Supervisor and department
Liu, Yang (Civil and Environmental Engineering)
Examining committee member and department
Buchanan, Ian (Civil and Environmental Engineering)
Zeng, Hongbo (Chemical and Materials Engineering)
Department
Department of Civil and Environmental Engineering
Specialization

Date accepted
2010-08-31T15:00:11Z
Graduation date
2010-11
Degree
Master of Science
Degree level
Master's
Abstract
The impact of cranberry juice was investigated with respect to the initial adhesion of three isogenic Burkholderia cepacia bacteria with different extracellular polymeric substance (EPS) producing capacities: a wild-type cepacian EPS producer PC184, mutant bacteria PC184rml with reduced EPS production, and PC184bceK with a deficiency in EPS production. Adhesion experiments demonstrated that in the presence of cranberry juice, the adhesive capacity of PC184 was largely reduced, while cranberry juice had little impact on the adhesion of either mutant. Thermodynamic modeling supported results from adhesion experiments. For PC184, the surface free energy change ΔGadh switched from negative in the absence of cranberry juice to positive when cranberry juice was added. Surface force apparatus (SFA) and scanning electron microscopy (SEM) studies demonstrated strong adsorption of cranberry juice components to bacterial EPS. It was concluded that cranberry juice components could impact bacterial initial adhesion by adhering to EPS and impairing bacterial adhesive capacity.
Language
English
DOI
doi:10.7939/R3TH40
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-04-29T15:47:17.490+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 3117349
Last modified: 2015:10:12 12:50:13-06:00
Filename: Yang_Xuejiao_Fall 2010.pdf
Original checksum: fca9760d7ceb5208c04440e95142b84d
Well formed: false
Valid: false
Status message: Unexpected error in findFonts java.lang.ClassCastException: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject cannot be cast to edu.harvard.hul.ois.jhove.module.pdf.PdfDictionary offset=2481
Page count: 91
Activity of users you follow
User Activity Date