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Permanent link (DOI): https://doi.org/10.7939/R3SG97

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Effects of macrophages and noggin suppression on the BMP-2-induced osteogenesis of human bone marrow mesenchymal stem cells Open Access

Descriptions

Other title
Subject/Keyword
bone morphogenetic protein-2
mesenchymal stem cells
macrophages
noggin
bone
osteogenesis
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Chen, Chao
Supervisor and department
Jiang, Hongxing (Surgery)
Examining committee member and department
Uludag, Hasan (Biomedical Engineering, Pharmacy)
Wang, Zhixiang (Cell Biology)
Department
Department of Surgery
Specialization

Date accepted
2011-01-06T22:00:21Z
Graduation date
2011-06
Degree
Master of Science
Degree level
Master's
Abstract
The osteogenic effects of bone morphogenetic protein-2 (BMP-2) on human mesenchymal stem cells (MSCs) are less profound than expected as compared with rodent cells, and supraphysiological dose of BMP-2 is required to achieve desired clinical outcome. The mechanism for this phenomenon is unclear. In this study, we examined the effects of macrophages and noggin suppression on the BMP-2-induced osteogenesis of human bone marrow MSCs in vitro. Our data show that macrophage conditioned medium significantly decreased the migration capacity, metabolic activity and BMP-2-induced osteogenesis of MSCs. In addition, knocking down noggin by small interfering RNA (siRNA) also significantly decreased BMP-2-induced osteogenesis and proliferation of MSCs. In summary, our studies demonstrated that macrophages and knocking down the expression of noggin decreased BMP-2-induced osteogenesis of human MSCs in vitro. In the future, manipulation on macrophage activation and noggin expression may allow us to achieve higher BMP-2-induced osteogenesis that leads to better bone healing.
Language
English
DOI
doi:10.7939/R3SG97
Rights
License granted by Chao Chen (cc6@ualberta.ca) on 2011-01-06T03:21:56Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: Microsoft Word - thesis final version Jan 5 2011.doc
File author: Harry Jiang
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