Role of CaMKII-alpha in the Neurodevelopment of Embryonic Zebrafish Open Access
- Other title
- Type of item
- Degree grantor
University of Alberta
- Author or creator
- Supervisor and department
Ali, Declan W. (Department of Biological Sciences)
- Examining committee member and department
Lukowiak, Kenneth D. (Department of Physiology and Pharmacology, University of Calgary)
Allison, William Ted (Department of Biological Sciences)
Nguyen, Peter V (Department of Physiology)
Funk, Gregory D (Department of Physiology)
Department of Biological Sciences
Physiology, Cell and Developmental Biology
- Date accepted
- Graduation date
Doctor of Philosophy
- Degree level
Calcium/calmodulin dependent protein kinase 2 (CaMKII) is a multifunctional serine/threonine kinase that is highly abundant in the central nervous system. It plays important roles in many neuronal functions, including neurite extension and maturation, activity-dependent synaptic plasticity and behavior. Although much focus has been given to elucidate the roles of this enzyme in activity-dependent synaptic plasticity, such as long term potentiation and depression, relatively little attention has been paid to explore the enzyme’s role in the synaptic receptor development in embryonic organism. Thus, my overall goal in this thesis is to examine the role CaMKII in the synaptic development of embryonic zebrafish. Zebrafish offers many advantages for developmental studies, rapid development and presence of identifiable Mauthner neurons (M-cells) in the embryos being two of them. Major findings reported in this thesis include (1) two days post fertilization (dpf) zebrafish M-cells predominantly express the alpha transcript of CaMKII genes, (2) knockdown of CaMKII alpha transcript led to increased branching of the ventral dendrite of the M-cells and aberrant locomotor response to touch, (3) development of excitatory synaptic receptor currents, specifically NMDA receptors were affected by knockdown of CaMKII-alpha transcript, while development of AMPA receptors were unaffected, and (4) knockdown of CaMKII-alpha transcript also affected the development of inhibitory GABA and glycine receptor currents. These findings contribute to our understanding of the important roles of CaMKII-alpha in the synaptic and neuronal development of embryonic organisms.
- Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
- Citation for previous publication
Roy,B., and Ali, D.W. (2013). Patch clamp recordings from embryonic zebrafish Mauthner cells. Journal of Visualized Experiments 79, e50551, doi:10.3791/50551.Roy, B., Ferdous, J., and Ali, D.W. (2015). NMDA receptors on zebrafish Mauthner cells require CaMKII-α for normal development. Developmental Neurobiology 75,145-62.Roy, B. and Ali, D.W. (2014). Multiple types of GABAA responses identified from zebrafish Mauthner cells. Neuroreport 25, 1232-6.
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