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Permanent link (DOI): https://doi.org/10.7939/R3KH3F

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HSV-1 VP11/12-mediated initiation of Src family kinase-PI3 kinase-Akt signalling Open Access

Descriptions

Other title
Subject/Keyword
SH2
herpes
Lck
src family kinase activation
HHV
peptide competition assay
VP11/12
HSV
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Wu, Frederick W
Supervisor and department
Smiley, James R (Medical Microbiology and Immunology)
Examining committee member and department
Barry, Michele (Medical Microbiology and Immunology)
Smiley, James R (Medical Microbiology and Immunology)
Ingham, Robert J (Medical Microbiology and Immunology)
Ostergaard, Hanne L (Medical Microbiology and Immunology; committee chair)
Shaw, Andrew R (Oncology)
Department
Department of Medical Microbiology and Immunology
Specialization
Virology
Date accepted
2013-07-31T10:34:02Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Herpes simplex virus type 1 is a ubiquitous human virus that causes cold sores and, increasingly, genital herpes. Upon virion-cell fusion, tegument proteins diffuse into host cytosol and, as such, are poised to remodel signalling pathways. Tegument proteins of interest here are virion protein 11/12 (VP11/12) and US3 protein kinase (US3PK), for its potential role in VP11/12 regulation. Wagner and Smiley (2011) posited a model wherein VP11/12 commandeers Lck to activate Akt. This study tests this model using various in vitro assays to dissect VP11/12 interaction with the Lck SH2 domain. My findings support the Wagner-Smiley model. To address curiosities in prior US3PK characterization, I generated US3-null mutants from wild-type and UL46-null viruses to produce US3- and UL46-null single and double knock-outs derived from KOS-37. Preliminary analysis of US3 mutants shows US3PK-dependent VP11/12 modification. These mutants allow for further exploration of US3PK-VP11/12 interplay.
Language
English
DOI
doi:10.7939/R3KH3F
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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