ERA

Download the full-sized PDF of Environmental biosafety of genetically engineered crops: Flax (Linum usitatissimum L.) as a model systemDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3132Z

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Environmental biosafety of genetically engineered crops: Flax (Linum usitatissimum L.) as a model system Open Access

Descriptions

Other title
Subject/Keyword
weed control
Biosafety
seed mediated
Genetically engineered crops
Gene flow
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Jhala, Amitkumar
Supervisor and department
Dr. Linda M Hall (Agricultural, Food and Nutritional Science)
Examining committee member and department
Dr. Jocelyn Hall (Biological Sciences)
Dr. Lloyd Dosdall (Agricultural, Food and Nutritional Science)
Dr. Randall Weselake (Agricultural, Food and Nutritional Science)
Dr. Robert Blackshaw (Agriculture and Agri-Food Canada, Lethbridge)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization

Date accepted
2009-12-23T18:29:20Z
Graduation date
2010-06
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Flax (Linum usitatissimum L.) is considered as a model plant species for multipurpose uses with whole plant utilization for several purposes including industril, food, animal feed, fiber, nutraceutical, pharmaceutical, and bioproduct markets. Therefore, flax is in the process of genetic engineering to meet the market requirements. Prior to commercial release of genetically engineered (GE) flax, a risk assessment was conducted to determine intra- and inter-specific pollen-mediated gene flow and for quantifing and mitigating the adventitious presence (AP) of volunteer flax in canola (Brassica napus L.). The results of pollen-mediated gene flow study (crop-to-crop) suggest that about 1.85% outcrossing would occur in adjunct area, when two flax cultivars were grown in close proximity of 0.1 m apart. Some rare gene flow events were recorded maximum up to 35 m distance from the pollen source but at a very low frequency. The genus Linum has several wild and weedy species, distributed in many parts of the world. A meta-analysis was conducted to determine the potential for gene introgression from GE flax to wild relatives, the occurrence, the phylogeny of flax wild relatives and reported interspecific hybridization. The results demonstrated that cultivated flax has ability to hybridize and form viable F1 plants with at least nine species of Linum; however, none of these species have been reported to occur in Canada. Hybridization of flax with many other wild relatives has either not been studied or reported. However, based on the evidence of reported work, gene flow from GE flax to wild or weedy relatives may occur elsewhere depending on species distribution, sympatry, concurrent flowering, ploidy level and sexual compatibility. The results of the experiments to mitigate the adventitious presence of flax volunteers in canola suggest that combinations of pre-plant followed by post-emergence herbicides were most effective for reducing volunteer flax density and AP in glufosinate-resistant canola. Post-emergence application of imazamox+imazethapyr, however, was not effective for controlling volunteer flax in imidazolinone-resistant canola. Best management practices were developed to mitigate transgene movement from GE flax to ensure co-existance of GE, conventional and organic flax without market harm.
Language
English
DOI
doi:10.7939/R3132Z
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2014-05-01T00:25:44.965+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1363166
Last modified: 2015:10:22 06:12:46-06:00
Filename: Jhala_Amitkumar_Spring 2010.pdf
Original checksum: 1948240c3f34c52dd8353b7ee843aaca
Well formed: true
Valid: false
Status message: Invalid page tree node offset=58685
Status message: Outlines contain recursive references.
File title: Linum hybrids among taxa with n=15���������������������������������..76
Activity of users you follow
User Activity Date