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Permanent link (DOI): https://doi.org/10.7939/R3H130

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The role of programmed death-1 (PD-1) expression in the negative selection of T lymphocytes Open Access

Descriptions

Other title
Subject/Keyword
thymocyte
PD-1
negative selection
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Parkman, Julia C
Supervisor and department
Baldwin, Troy (Medical Microbiology and Immunology)
Examining committee member and department
Ostergaard, Hanne (Medical Microbiology and Immunology)
Ingham, Robert (Medical Microbiology and Immunology)
Anderson, Colin (Surgery)
Department
Department of Medical Microbiology and Immunology
Specialization

Date accepted
2010-12-14T17:54:30Z
Graduation date
2011-06
Degree
Master of Science
Degree level
Master's
Abstract
The immune system must be able to mount a response against pathogens and transformed cells while remaining tolerant to healthy host tissue. A key process for ensuring this self-tolerance is the negative selection of self-reactive thymocytes. Expression of Programmed Death-1 (PD-1), a co-inhibitory member of the CD28 family associated with dampened peripheral immune responses,was found to be upregulated in 20-40% of thymocytes undergoing negative selection in the HYcd4model of thymic development. Although analysis of gene and protein expression directly ex vivo indicates that PD-1- and PD-1+ thymocytes are equally apoptotic, PD-1+ thymocytes appear to be protected from apoptosis in an in vitro stimulation assay. Analysis of HYcd4PD-1-/- mice indicates that thymocytes receive a higher intensity signal in the absence of PD-1. Future work utilizing HYcd4PD-1-/- mice will increase our understanding of the role of PD-1 in thymic negative selection.
Language
English
DOI
doi:10.7939/R3H130
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: THESIS-PRE
File author: Julia Parkman
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