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Permanent link (DOI): https://doi.org/10.7939/R3RH64

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Flapwing Dephosphorylates Merlin and Moesin and Regulates Epithelial Integrity in Drosophila Open Access

Descriptions

Other title
Subject/Keyword
Merlin
Moesin
epithelial integrity
dephosphorylation
Drosophila
flapwing
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Yang, Yang
Supervisor and department
Hughes, Sarah (Department of Medical Genetics, Department of Cell Biology)
Examining committee member and department
Godbout, Roseline (Department of Oncology)
Holmes, Charles (Department of Biochemistry)
Hughes, Sarah (Department of Medical Genetics, Department of Cell Biology)
LaPointe, Paul (Department of Cell Biology)
Department
Department of Cell Biology
Specialization

Date accepted
2013-09-26T07:59:28Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Merlin is a tumor suppressor protein whose inactivation is associated with familial Neurofibromatosis Type II (NF2) and other sporadic tumors. The growth-suppressive function of Merlin is modulated by reversible phosphorylation. Our previous finding showed the Sterile20 kinase Slik coordinately regulates the activity of Merlin and Moesin in Drosophila melanogaster. Here I report that the Drosophila protein phosphatase 1β flapwing (flw) is involved in the coordinate dephosphorylation of Merlin and Moesin. Flw forms a complex with Merlin and Moesin. Changes in flw expression level lead to corresponding changes in the phosphorylation pattern of Merlin and Moesin. Moreover, reducing flw expression in epithelial cells results in increased membrane-association of Merlin and Moesin and disruption of epithelial integrity. Taken together, flw plays a role in epithelial organization by regulating dephosphorylation of Merlin and Moesin.
Language
English
DOI
doi:10.7939/R3RH64
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Yang Y, Primrose DA, Leung AC, Fitzsimmons RB, McDermand MC, Missellbrook A, Haskins J, Smylie AS, Hughes SC (2012) The PP1 phosphatase flapwing regulates the activity of Merlin and Moesin in Drosophila. Dev Biol 361: 412-426

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File title: Microsoft Word - Thesis Final Yang_Yang_Fall 2013.docx
File author: Yang Yang
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