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Clinical transitions in photoacoustic imaging Open Access


Other title
clinical imaging
photoacoustic imaging
coded excitation
quantitative imaging
gene expression
Type of item
Degree grantor
University of Alberta
Author or creator
Harrison, Tyler J
Supervisor and department
Zemp, Roger (Electrical and Computer Engineering)
Examining committee member and department
Becher, Harald (Medicine)
Tsui, Ying (Electrical and Computer Engineering)
Zemp, Roger (Electrical and Computer Engineering)
Van, Vien (Electrical and Computer Engineering)
Tavakoli, Mahdi (Electrical and Computer Engineering)
Joseph, Dileepan (Electrical and Computer Engineering)
Foster, F. Stuart (Department of Medical Biophysics, University of Toronto)
Department of Electrical and Computer Engineering
Biomedical Engineering
Date accepted
Graduation date
Doctor of Philosophy
Degree level
Optical imaging has long been a gold standard for medical imaging. However, due to the high optical scattering in tissues, it has not been possible to image deeper than a few wavelengths with a useful resolution. Photoacoustic imaging - the use of short-pulsed lasers to induce an ultrasound signal - can provide optical contrast at ultrasonic resolution. This thesis focuses on the refinement of photoacoustic imaging for practical use by clinicians in clinical and preclinical studies. The first focus of this work is in system development. Beginning with a single-element based system suitable for some preclinical studies, we have worked towards a more practical ultrasound array based system. This system provides several different ultrasound modes to augment the photoacoustic data available including ultrasound flash imaging, synthetic aperture imaging, and ultrasound Doppler imaging. With this array system we demonstrate two fundamentally different clinically-applicable photoacoustic techniques using both optical and acoustic resolution. Similar methods may be used with both of these using multiple interrogation wavelengths providing label-free functional imaging. Other system development work has focused on transducer design and fabrication to provide better imaging capabilities. The second focus of this thesis is image reconstruction. We explore alternative imaging techniques using different source/array geometries and illumination patterns, demonstrate the applicability of ultrasound beamformers to photoacoustic imaging, and even work with different ultrasound imaging techniques including s-sequence excitation and minimum variance beamforming. The final focus is on clinical applications. We show photoacoustic imaging of gene expression through a tyrosinase reporter gene developed by my colleagues with exciting applications in studying metabolic pathways and cancer development. Using a longer wavelength, we also demonstrate the imaging of small metallic seeds used in brachytherapy which may aid in localizing them during radiotherapy of prostate cancer. Finally, we demonstrate multi-modal human imaging of the thyroid in vivo. Through exploration of system development, image reconstruction, and applications, this thesis aims to prove the clinical practicality of photoacoustic imaging.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
T. Harrison, J. Ranasinghesagara, H. Lu, K. Mathewson, A. Walsh, and R. Zemp, "Combined photoacoustic and ultrasound biomicroscopy,'' Opt. Express 17, 22041-22046 (2009).T. Harrison, D. Jeffery, E. Wiebe, and Roger J. Zemp, "Real-time clinically oriented array-based in vivo combined photoacoustic and power Doppler imaging.'' Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89435A (March 3, 2014), doi:10.1117/12.2040609.Tyler Harrison, Parsin Hajireza, Alexander Forbrich, Roger Zemp, "Optical-resolution photoacoustic micro-endoscopy with ultrasound array system detection .'' Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 85810C (March 4, 2013), doi:10.1117/12.2004887.Harrison, T., Zemp, R.J., "The applicability of ultrasound dynamic receive beamformers to photoacoustic imaging,'' Ultrasonics, Ferroelectrics and Frequency Control, IEEE Transactions on , vol.58, no.10, pp.2259,2263, October 2011 doi: 10.1109/TUFFC.2011.2076.T. Harrison, P. Shao, and R. Zemp, "A least-squares fixed-point iterative algorithm for multiple illumination photoacoustic tomography,'' Biomed. Opt. Express 4, 2224-2230 (2013).Harrison, T., Sampaleanu, A., Zemp, R.J., "S-sequence spatially-encoded synthetic aperture ultrasound imaging,'' Ultrasonics, Ferroelectrics and Frequency Control, IEEE Transactions on , vol.61, no.5, pp.886,890, May 2014 doi: 10.1109/TUFFC.2014.6805701.T. Harrison, P. Shao, and R. Zemp, "S-sequence spatial coding for iterative multiple illumination photoacoustic tomography,'' J. Biomed. Opt. 140294P.Harrison T, Zemp RJ, "Coregistered photoacoustic-ultrasound imaging applied to brachytherapy.'' J. Biomed. Opt. 16(8):080502-080502-3. doi:10.1117/1.3606566.Tyler Harrison, Robert J. Paproski, Roger J. Zemp, "In vivo imaging of inducible tyrosinase gene expression with an ultrasound array-based photoacoustic system.'' Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82230S (February 9, 2012), doi:10.1117/12.908987.

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