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Isolation and fast analysis of phytochemical constituents in Echinacea species and Rhodiola rosea L. using high-speed counter-current chromatography and ultra fast liquid chromatography-mass spectrometry Open Access


Other title
ultra-fast liquid chromatography
rhodiola rosea
high-speed counter-current chromatography
Type of item
Degree grantor
University of Alberta
Author or creator
Mudge, Elizabeth M
Supervisor and department
Schieber, Andreas (Agricultural Food and Nutritional Science)
Examining committee member and department
Curtis, Jonathan (Agricultural Food and Nutritional Science)
Cairo, Christopher (Chemistry)
Department of Agricultural, Food, and Nutritional Science
Food Science and Technology
Date accepted
Graduation date
Master of Science
Degree level
High-speed counter-current chromatography was used for the purification of phytochemical components from the roots of Echinacea angustifolia (DC.) Hell and Rhodiola rosea L. Five alkylamides were purified from Echinacea angustifolia roots using two solvent systems and seven phenylalkanoid and monoterpene glycosides were isolated from Rhodiola rosea roots using one solvent system and semi-preparative HPLC. Fast analytical methods were developed for the identification of alkylamides in Echinacea roots and commercial products available in the Canadian marketplace. 24 alkylamides were identified in 15 minutes using ultra-fast liquid chromatography with diode array and mass spectrometric detection. The three major alkylamides obtained by HSCCC were used as quantitative standards to determine alkylamide contents in different products. Also, a 22 minute method using UFLC-DAD-MS was developed for the characterization of 27 components in Rhodiola rosea roots. These techniques can be used in quality and authenticity control of natural health products containing Echinacea and Rhodiola rosea.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Lopes-Lutz, Daise (2011) J. Agric. Food Chem. 59 (2) 491-494 (ACS publications)
, Elizabeth (2011) J. Agric. Food Chem. 59 (15) 8086–8094 (ACS Publications)

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File title: Thesis July 27 2011
File author: Elizabeth Mudge
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