ERA

Download the full-sized PDF of Oral transmissibility of prion disease is enhanced by binding to soil particlesDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R34T6F57N

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Biological Sciences, Department of

Collections

This file is in the following collections:

Journal Articles (Biological Sciences)

Oral transmissibility of prion disease is enhanced by binding to soil particles Open Access

Descriptions

Author or creator
Johnson, C.J.
Pedersen, J.A.
Chappell, R.J.
McKenzie, D.
Aiken, J.M.
Additional contributors
Subject/Keyword
incubation periods
Chronic Wasting Disease
Scrapie virus
Crohns-Disease
detergent extraction
Bovine Spongiform Encephalopathy
mule deer
mink encephalopathy
clay-minerals
inorganic microparticles
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
Soil may serve as an environmental reservoir for prion infectivity and contribute to the horizontal transmission of prion diseases (transmissible spongiform encephalopathies [TSEs]) of sheep, deer, and elk. TSE infectivity can persist in soil for years, and we previously demonstrated that the disease-associated form of the prion protein binds to soil particles and prions adsorbed to the common soil mineral montmorillonite (Mte) retain infectivity following intracerebral inoculation. Here, we assess the oral infectivity of Mte- and soil-bound prions. We establish that prions bound to Mte are orally bioavailable, and that, unexpectedly, binding to Mte significantly enhances disease penetrance and reduces the incubation period relative to unbound agent. Cox proportional hazards modeling revealed that across the doses of TSE agent tested, Mte increased the effective infectious titer by a factor of 680 relative to unbound agent. Oral exposure to Mte-associated prions led to TSE development in experimental animals even at doses too low to produce clinical symptoms in the absence of the mineral. We tested the oral infectivity of prions bound to three whole soils differing in texture, mineralogy, and organic carbon content and found soil-bound prions to be orally infectious. Two of the three soils increased oral transmission of disease, and the infectivity of agent bound to the third organic carbon-rich soil was equivalent to that of unbound agent. Enhanced transmissibility of soil-bound prions may explain the environmental spread of some TSEs despite the presumably low levels shed into the environment. Association of prions with inorganic microparticles represents a novel means by which their oral transmission is enhanced relative to unbound agent.
Date created
2007
DOI
doi:10.7939/R34T6F57N
License information
Rights
© 2007 Johnson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation for previous publication
Johnson CJ, Pedersen JA, Chappell RJ, McKenzie D, Aiken JM (2007) Oral transmissibility of prion disease is enhanced by binding to soil particles. PLoS Pathog 3(7): e93. doi:10.1371/journal.ppat.0030093
Source
Link to related item

File Details

Date Uploaded
Date Modified
2014-04-30T21:57:56.041+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 384740
Last modified: 2015:10:12 16:24:53-06:00
Filename: PLOS_Path_3_2007_e93.pdf
Original checksum: bf4c6afc8fd5c8cceeea2a742c59256b
Well formed: true
Valid: true
File title: untitled
Page count: 8
Activity of users you follow
User Activity Date