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Permanent link (DOI): https://doi.org/10.7939/R3VH5CS2H

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Development and Analysis of Magnetic Carbon Nanotube and Microbubble-Assisted High Centrifugation Field Techniques For The Increase of Cell Membrane Permeability Open Access

Descriptions

Other title
Subject/Keyword
Centrifugation
Cell Membrane
Permeability
Microbubble
Finite Element Analysis
Interaction
Carbon Nanotube
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
He, Chuan
Supervisor and department
Chen, Jie
Examining committee member and department
Chen, Jie (Electrical and Computer Engineering)
Zeng, Hongbo (Chemical and Materials Engineering)
Datla, Raju (National Research Council)
Zhao, Vicky (Electrical and Computer Engineering)
Dew, Steve (Electrical and Computer Engineering)
Department
Department of Electrical and Computer Engineering
Specialization
Biomedical Engineering
Date accepted
2014-01-02T13:49:16Z
Graduation date
2014-06
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
To cure diseases at the gene level, therapeutic biomaterials need to enter the cells. However, since the cell membrane is a highly selective structure, its permeability is usually very low. In this thesis, we propose two innovative and efficient methods to increase cell membrane permeability. The first method is based on magnetic carbon nanotubes (mCNT). By using atomic force microscope force curve analysis (AFM-FCA) and transmission electron microscope (TEM) images, we successfully develop mCNT from raw materials. Under a magnetic field, mCNT can facilitate cell endocytosis by firmly attaching onto the cell membrane. As a result, the cell membrane permeability of several mammalian cell lines is increased. The second method to increase cell membrane permeability is based on using microbubble-assisted high centrifugation field. The mechanism of this method relies on the centrifugation-induced collision between microbubbles and cells and the force of the microbubbles bursting. The validity of this method is demonstrated on mammalian cells and plant cells. Theoretical models are built to simulate the interaction between microbubbles and cells in the centrifugation field. The simulation results indicate that intracellular pathways can be created once the relative velocity between the microbubble and cell is beyond a critical value. In addition, cell deformed morphology induced in the centrifugation field and cell mechanical properties are closely related to the resulting increase of cell membrane permeability.
Language
English
DOI
doi:10.7939/R3VH5CS2H
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
He, Chuan; Hao, Yuzhi; Zeng, Hongbo; et al., Separation and purification technology [1383-5866] yr:2011 vol:81 iss:2 pg:174-183He, Chuan; Gu, Quanrong; Zeng, Hongbo; et al., Cellular and molecular bioengineering [1865-5025] yr:2013 vol:6 iss:3 pg:266-278He, Chuan; Gu, Quanrong; Huang, Min; et al., FEBS letters [0014-5793] yr:2013 vol:587 iss:3 pg:285-90

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