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Permanent link (DOI): https://doi.org/10.7939/R3R07B

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Genetic Analysis of Lignification and Secondary Wall Development in Bast Fibers of Industrial Hemp (Cannabis sativa) Open Access

Descriptions

Other title
Subject/Keyword
Secondary Wall Development
Bast Fiber
Virally Induced Gene Silencing
Cannabis sativa
Lignification
Industrial Hemp
Phenylpropanoid pathway
VIGS
Microarray
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Koziel, Susan P.
Supervisor and department
Deyholos, Michael (Biological Sciences)
Examining committee member and department
Gallin, Warren J. (Biological Sciences)
Strelkov, Stephen (Faculty of Agricultural, Food and Nutritional Science)
Scarpella, Enrico (Biological Sciences)
Department
Department of Biological Sciences
Specialization

Date accepted
2009-10-15T21:20:59Z
Graduation date
2010-06
Degree
Master of Science
Degree level
Master's
Abstract
Industrial hemp (Cannabis sativa) is a highly productive crop that is well suited to cultivation in Canada. To better understand the development of bast (phloem) fiber secondary walls and to facilitate reverse genetics screening for improved germplasm, I undertook two sets of microarray experiments. The first compared transcript expression in stem peels at three positions along the length of the stem. The second set of microarray experiments compared transcript expression in adjacent tissue layers along the radial axis of the stem. The transcripts that were enriched in fiber-producing tissues in both studies were consistent with a dynamic program of cell wall deposition. Detailed qRT-PCR analysis of specific lignification genes identified the best targets for reverse genetics. Finally, as a first step towards establishing a virally induced gene silencing (VIGS) system, I identified viruses that produced visual symptoms of infection, although qRT-PCR failed to confirm the infection
Language
English
DOI
doi:10.7939/R3R07B
Rights
License granted by Susan Koziel (skoziel@ualberta.ca) on 2009-10-13T15:05:30Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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