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Permanent link (DOI): https://doi.org/10.7939/R3MX48

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Establishing the anti-cancer effects of unsaturated fatty acids and a novel oil on human breast cancer cells Open Access

Descriptions

Other title
Subject/Keyword
cell membrane
n-3 fatty acids
unsaturated fatty acids
breast cancer
novel
conjugated linoleic acid
anti-cancer
n-6 fatty acids
phospholipids
oil
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Yu, Howe-Ming
Supervisor and department
Field, Catherine (AFNS)
Examining committee member and department
Mazurak, Vera (AFNS)
Weselake, Randall (AFNS)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization
Nutrition & Metabolism
Date accepted
2012-09-18T15:48:33Z
Graduation date
2012-09
Degree
Master of Science
Degree level
Master's
Abstract
N-3 and n-6 fatty acids and conjugated linoleic (CLA) acid have been known to inhibit breast cancer cell growth, however, effects on normal cells and of single and mixtures of n-3 and n-6 pathway intermediates have not been thoroughly investigated. The objective of this thesis was to determine the effects of fatty acids with potential anti-cancer activity and a fatty acid mixture, representing a new plant source, on breast cell viability and cell membrane composition. Human tumorigenic MDA-MB-231 and MCF-7 cells lines and a non-tumorigenic MCF-12A cell line were studied. All fatty acids, including the mixture representing a stearidonic acid (SDA) enriched flax oil (SO) reduced the viability of tumorigenic but not non-tumorigenic cells, compared to cells without fatty acids (p<0.05). α-linolenic acid, docosahexaenoic acid (DHA), linoleic acid, and arachidonic acid (AA) from treatments were minimally converted down their respective pathways and incorporated into membranes as the original fatty acid. Intermediates SDA, eicosatetraenoic acid and eicosapentaenoic acid showed significant conversion down the n-3 pathway, but not to DHA. N-6 γ-linolenic acid and dihomo-γ-linolenic acid (DGLA) treatments accumulated DGLA in the phospholipids, but not AA. SO treatment showed conversion to docosapentaenoic acid and DGLA in tumorigenic cell lines, but was not well converted in MCF-12A cells. C9, t11-CLA decreased tumorigenic growth (p<0.05) and was isomerized to a more potent isomer on cell viability. Overall, our data demonstrates that in vitro, n-3 and n-6 fatty acid intermediates, CLA and a n-3 and n-6 mixture decrease tumorigenic cell viability and are incorporated into membranes with significant conversion down their pathways. Inhibitory effects of n-3 and n-6 fatty acid intermediates seem to be independent of conversion to biological endpoints, and c9, t11-CLA’s inhibitory effects may be due to its isomerization.
Language
English
DOI
doi:10.7939/R3MX48
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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