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Permanent link (DOI): https://doi.org/10.7939/R3NX4K

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The Role of Autotaxin in the Regulation of Lysophosphatidylcholine-Induced Cell Migration Open Access

Descriptions

Other title
Subject/Keyword
LPA
Breast Cancer
ATX
Metastasis
Lysophosphatidic adic
Lysophosphatidylcholine
Melanoma
Migration
Autotaxin
LPC
Lysophosphatidate
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Gaetano, Cristoforo Giuseppe
Supervisor and department
Dr. Brindley, David N. (Biochemisty)
Examining committee member and department
Dr. McMullen, Todd (Medicine)
Dr. Holmes, Charles F. (Biochemisty)
Dr. Michalak, Marek (Biochemistry)
Department
Department of Biochemistry
Specialization

Date accepted
2010-04-08T16:35:17Z
Graduation date
2010-06
Degree
Master of Science
Degree level
Master's
Abstract
Increased expression of autotaxin has been shown to promote metastasis formation and cancer proliferation. These actions could be related to the catalytic activity of autotaxin which converts lysophosphatidylcholine into lysophosphatidate extracellularly or non-catalytic functions of autotaxin may be responsible. Also both LPC and LPA have been reported to stimulate migration through their respective receptors. This work investigates the role of autotaxin in controlling the motility of two cancer cell lines. With the use of autotaxin inhibitors we were able to block LPC-induced migration. Knocking-down autotaxin secretion also blocked stimulation of migration by LPC. Autotaxin inhibitors abolished any migratory effects from media collected from autotaxin secreting cells. We determined that LPC alone is unable to stimulate migration. Also we did not observe non-catalytic effects of autotaxin on migration. This thesis provides strong evidence that the inhibition of autotaxin production or activity would provide a beneficial therapy in the prevention of tumour growth or metastasis in patients with autotaxin expressing tumours.
Language
English
DOI
doi:10.7939/R3NX4K
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: Cris Gaetano - Masters Thesis for PDF
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