Download the full-sized PDF
Permanent link (DOI): https://doi.org/10.7939/R3JQ0T19S
This file is in the following communities:
|Biological Sciences, Department of|
This file is in the following collections:
|Journal Articles (Biological Sciences)|
Gawky Is a Component of Cytoplasmic mRNA Processing Bodies Required for Early Drosophila Development Open Access
- Author or creator
Schneider, M. D.
Pare, J. M.
Hobman, T. C.
Simmonds, A. J.
- Additional contributors
- Type of item
- Journal Article (Published)
In mammalian cells, the GW182 protein localizes to cytoplasmic bodies implicated in the regulation of messenger RNA ( mRNA) stability, translation, and the RNA interference pathway. Many of these functions have also been assigned to analogous yeast cytoplasmic mRNA processing bodies. We have characterized the single Drosophila melanogaster homologue of the human GW182 protein family, which we have named Gawky (GW). Drosophila GW localizes to punctate, cytoplasmic foci in an RNA-dependent manner. Drosophila GW bodies (GWBs) appear to function analogously to human GWBs, as human GW182 colocalizes with GW when expressed in Drosophila cells. The RNA-induced silencing complex component Argonaute2 and orthologues of LSm4 and Xrn1 (Pacman) associated with 5' - 3' mRNA degradation localize to some GWBs. Reducing GW activity by mutation or antibody injection during syncytial embryo development leads to abnormal nuclear divisions, demonstrating an early requirement for GWB-mediated cytoplasmic mRNA regulation. This suggests that gw represents a previously unknown member of a small group of genes that need to be expressed zygotically during early embryo development.
- Date created
- License information
- This article is available under the Creative Commons Attribution-NonCommercial-ShareAlike license (CC BY-NC-SA). This license lets others remix, tweak, and build upon your work non-commercially, as long as they credit you and license their new creations under the identical terms.
- Citation for previous publication
Schneider, M.D., Najand, N., Chaker, S., Pare, J. M., Haskins, J., Hughes, S. C., Hobman, T. C., Locke, J., & Simmonds, A. J. (2006). Gawky Is a Component of Cytoplasmic mRNA Processing Bodies Required for Early Drosophila Development.
The Journal of Cell Biology , 174(3), 349-358. DOI: 10.1083/jcb.200512103.
- Link to related item
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 3089914
Last modified: 2015:10:12 18:31:00-06:00
Original checksum: 40b8d7211b2283cb221ea3713eeaec7d
Well formed: true
File title: untitled
Page count: 10