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Evaluation of Residual Starch Determination Methods for Dried Distillers' Grains with Solubles (DDGS) Open Access


Other title
Residual starch
Dried distillers’ grains with solubles (DDGS)
Starch determination
Raw starch hydrolysis
Type of item
Degree grantor
University of Alberta
Author or creator
Reed, Desmond K
Supervisor and department
Vasanthan, Thava (AFNS)
Bressler, David (AFNS)
Examining committee member and department
Vasanthan, Thava (AFNS)
Bressler, David (AFNS)
Sauvageau, Dominic (Chemical and Materials Engineering)
Department of Agricultural, Food, and Nutritional Science

Date accepted
Graduation date
Master of Science
Degree level
A co-product of ethanol production is known as dried distillers’ grains with solubles (DDGS). DDGS contains residual starch that escaped hydrolysis under ideal conditions during ethanol production; therefore, residual starch in DDGS is expected to include enzyme-resistant starch. Common starch analysis methods involve enzymatic or chemical hydrolysis, but these methods may be unable to access resistant starch leading to an underestimation of the residual starch in DDGS. This study used several enzymatic starch analysis methods, with or without chemical pre-treatment, in an attempt to find an appropriate methodology for the measurement of residual starch in DDGS. Alternatively, an acid hydrolysis method that is commonly used by industry was also attempted for comparison. It was determined that the addition of a chemical solubilization step prior to enzymatic hydrolysis was necessary to access resistant starch present in DDGS samples. Chemical hydrolysis using dilute acid vastly overestimated the amount of residual starch in DDGS.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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